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Plasmid#154195PurposeLentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)DepositorInsertCaTCH BC1
UseLentiviral; Catch barcode labelingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaTCH_empty-backbone
Plasmid#157746PurposeLentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequencesDepositorTypeEmpty backboneUseLentiviral; Catch barcode cloningTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2
Plasmid#118000PurposeCo-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.DepositorInsertDerlin1-mOrange2
UsePlant/bacteria binary vectorTagsExpressionPlantMutationPromoterUbiquitin-10Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a-EGFP
Plasmid#205311PurposeExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a-EGFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
P12p-bio
Plasmid#47726PurposeExpresses enzymatically monobiotinylated full-length P12p ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P12p
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RAP3-bio
Plasmid#47796PurposeExpresses enzymatically monobiotinylated full-length RAP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RAP3
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP2-bio
Plasmid#47737PurposeExpresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP2
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-WHaloCaMP1a-EGFP
Plasmid#205307PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
UseTagsExpressionBacterialMutationPromoterPosmY, PsulA, and grpEAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a-EGFP
Plasmid#205309PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
AMA1-bio
Plasmid#47741PurposeExpresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AMA1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBA140-bio
Plasmid#47742PurposeExpresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBA140
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
P12-bio
Plasmid#47725PurposeExpresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P12
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RAP1-bio
Plasmid#47794PurposeExpresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RAP1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only