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Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
sox10:PDGFRA mutant
Plasmid#98697PurposeI-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafishDepositorInsertPDGFRA (PDGFRA Human)
UseZebrafish expressionTagsExpressionMutationexon 8 and 9 deletionPromoterAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MO70-hJunctate - EF-mutant
Plasmid#79597PurposeTransient and retroviral expressionDepositorInsertjunctate (ASPH Human)
UseRetroviralTagsFLAGExpressionMammalianMutation77DAD79 to 77AAA79PromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW1-mutant
Plasmid#17795DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 to Alanine; Proline 202 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP2-WW2-mutant
Plasmid#17796DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 258 to Alanine Proline 261 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-Af-mut
Plasmid#120829PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5 V114M/V872M - "african mut"
UseTagsV5ExpressionMammalianMutationV114M/V872MPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mut mod
Plasmid#48165Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Mut (Mut Mouse)
UseLuciferaseTagsExpressionMutationA/T mutation in the ATG of the uORFPromoterAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1mut
Plasmid#86690PurposeFor transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
UseTagsHAExpressionMutationmutated region NHDC (asparagine 273 to cysteine 2…PromoterT3Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsHis6x and mGFPmut3ExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 R mut
Plasmid#27159PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationR box mutant: K249A/V250A/E251APromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9G(RBSmut)_arr2
Plasmid#149578Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2 HNF4A P2 mut
Plasmid#60325PurposeMutated HNF4A P2 promoter region cloned into the pGL2-basic backbone. The -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.DepositorInsertHNF4A P2 mut (-181 G to A) (HNF4A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationThe -181 G to A mutation is in a HNF1alpha bindin…PromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HIRAmut-polyA
Plasmid#59782PurposeThis Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).DepositorInsertHIRA
UseTagsExpressionMammalianMutationChange from 'GATCGAAGTTTG' to 'GAc…PromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K(RBSmut)_arr2
Plasmid#149577Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 5
Plasmid#8479DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationG192DPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
MLS-mir-17-92-Mut20
Plasmid#64095Purposeexpresses a mutated version of mir-17-92 (mutation of miR-20 seed)DepositorInsertmiR-17-92 (MIR17HG Human)
UseTagsExpressionMammalianMutationmutation of miR-20 seed sequencePromoterMSCVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-3'ss mut
Plasmid#67644PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation3' splice site muattionPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSP1-mp53AS-6xMUT
Plasmid#20904DepositorInsertp53AS (Trp53 Mouse)
UseTagsExpressionMammalianMutationpoint mutation (Ser/Thr-Ala) of the 6 N-terminal …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only