We narrowed to 747 results for: PI
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Plasmid#227666PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertAmR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-6
Plasmid#227665PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-4
Plasmid#227664PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-2
Plasmid#227663PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionBacterialMutationPromoterp14G-BCD2Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-8
Plasmid#227670PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.DepositorInsertAmR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-4
Plasmid#227668PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.DepositorInsertSmR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pS44i8GH-1
Plasmid#207525PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH-1699
Plasmid#124227PurposeMammalian SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and noneAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-N-Ezrin
Plasmid#202684Purposefluorescent protein tagged ezrinDepositorInsertMus musculus EZR(1-586):EGFP (Ezr Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTE4398
Plasmid#74042PurposeExpresses human codon-optimized LbCpf1 and Lb crRNA.DepositorInsertsLb crRNA
LbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVE13300
Plasmid#137715PurposeExpresses impLbCas12a in mammalian cells.DepositorInsertimpLbCas12a
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PLCdelta-PH
Plasmid#21179Purposeencodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipidsDepositorInsertPLCdelta (PLCD1 Human)
UseTagsGFPExpressionMammalianMutationPH domain of human PLC delta, amino acids Met1–Il…PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pS148∙CsR
Plasmid#197858PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Ap/Cb resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasso
Plasmid#207530PurposeConditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgDepositorInsertcytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only