We narrowed to 3,820 results for: SPL;
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Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)
Plasmid#107860PurposeTo quickly insert CTD mutants into TDP43 splicing reporterDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of CTD in TDP43 splicing reporter, inser…PromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)
Plasmid#107855PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W and W385G in splicing reporterPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
KHBD00510
Plasmid#39611DepositorInsertCG8365 (E(spl)m8-HLH Fly)
UseGateway donr vectorTagsExpressionMutationPromoternoneAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00413
Plasmid#34520DepositorInsertCG8365 (E(spl)m8-HLH Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00021
Plasmid#34183DepositorInsertCG14548 (E(spl)mbeta-HLH Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00026
Plasmid#34188DepositorInsertCG8333 (E(spl)mgamma-HLH Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00016
Plasmid#34178DepositorInsertCG8361 (E(spl)m7-HLH Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFOSGE_COF2B09
Plasmid#70918PurposeGateway entry cloneDepositorInsertgro (gro Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA
Plasmid#216319PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Cerulean + splice acceptor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
UseTagsExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only