We narrowed to 549 results for: mak
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Plasmid#149540PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
ExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD3-2A_pMI-LO
Plasmid#153418PurposeRetroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrangeDepositorInsertmurine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
UseRetroviralExpressionMammalianAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HOXB7 (human) pCMV
Plasmid#8539DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHOXB7 (HOXB7 Human)
TagsFLAGExpressionMammalianMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…Available sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSIN-hSNCA-NE
Plasmid#102366PurposeLentiviral overexpression of synuclein alphaDepositorInsertsynuclein alpha (SNCA Human)
UseLentiviralTagsNEExpressionMammalianMutationA silent mutation (333bp downstream of ATG) was i…PromoterEF-1aAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ132-tRNA
Plasmid#179211PurposeGolden Gate entry vector; 2nd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pERB215
Plasmid#61499PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHaloTag-GFP-Nuf2
mCherry-DHFR
TagsHaloTag and mCherryExpressionMammalianAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HOXB3 (human) FLAG pSP65
Plasmid#8523DepositorInsertHOXB3 (HOXB3 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-BFP
Plasmid#162755PurposeRetroviral (MSCV) expression of mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseRetroviralExpressionMammalianAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
TagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,+)-Fc-His
Plasmid#72147PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-GFP
Plasmid#130977PurposeExpression of KIF1C-GFP in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1C-GFP (KIF1C Human)
TagsGFPExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ133-tRNA
Plasmid#179212PurposeGolden Gate entry vector; 3rd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D
Plasmid#69289PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,+)-AP-His
Plasmid#72021PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSema3f (Sema3f Mouse)
TagsAP-HisExpressionMammalianPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-E2Crimson
Plasmid#162756PurposeRetroviral (MSCV) expression of E2-Crimson fluorescent proteinDepositorInsertE2-Crimson
UseRetroviralExpressionMammalianAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only