We narrowed to 1,322 results for: pET28a

1. pET28a-SUMO-DogTag

   Plasmid

   #105629

   Purpose

   Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   SUMO-DogTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-His-hAos1

   Plasmid

   #53135

   Purpose

   E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Aos1 (SAE1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-eGFP-MinD

   Plasmid

   #133622

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

   Depositor

   Petra Schwille

   Article

   Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

   Insert

   EGFP-MinD (minD E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6his-WT gusA-pET28a+

   Plasmid

   #61156

   Purpose

   N -terminal His tagged wild-type GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   gusA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Use

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mH6-Cas12c2

   Plasmid

   #120873

   Purpose

   Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-TEV-TBP6.9F34MF37MF77M

   Plasmid

   #168268

   Purpose

   Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   TBP6.9
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F34M, F37M and F77M

   Promoter

   T7 promotor

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-LgBiT-SpyCatcher

   Plasmid

   #223634

   Purpose

   Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6, LgBiT

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. BARD1 26-327 pET28a

    Plasmid

    #12646

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-mH6-Cas12g1

    Plasmid

    #120879

    Purpose

    Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12g1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SpyCatcher-mi3

    Plasmid

    #112255

    Purpose

    Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    SpyCatcher-mi3
    

    Use

    Tags

    C-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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