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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,378 results for: pho;

Showing: 321 - 340 of 9378 results

  1. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181950-plasmid-map-sequence-id-356579

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  4. pCapV

    Plasmid
    #183028
    Purpose
    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase CapV
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD promoter
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183028-plasmid-map-sequence-id-361615

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  5. pNAS1b split mCherry NEDD4 WW2 neg ctrl

    Plasmid
    #112030
    Purpose
    Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112030-plasmid-map-sequence-id-217140

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  6. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid
    #107163
    Purpose
    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme
    Depositor
    Jose Carlos Cameselle
    Article
    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.
    Insert
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available Since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107163-plasmid-map-sequence-id-204806

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  7. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  9. pCapVQ329R

    Plasmid
    #183029
    Purpose
    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase variant CapVQ329R
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    glutamine 329 changed to arginine
    Promoter
    pBAD promoter
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183029-plasmid-map-sequence-id-361616

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  12. p20StAD

    Plasmid
    #190268
    Purpose
    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.
    Depositor
    Assaf Mosquna
    Article
    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2
    Type
    Empty backbone
    Use
    Synthetic Biology; Yeast genome-integrating vector
    Tags
    GAL4-AD
    Expression
    Mutation
    Promoter
    TDH3
    Available Since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    190268-plasmid-map-sequence-id-377252

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  13. p21StBD

    Plasmid
    #190269
    Purpose
    Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.
    Depositor
    Assaf Mosquna
    Article
    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2
    Type
    Empty backbone
    Use
    Synthetic Biology; Yeast genome-integrating vector
    Tags
    GAL4-BD
    Expression
    Mutation
    Promoter
    TDH3
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190269-plasmid-map-sequence-id-377250

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  15. pAtVPZv1.0

    Plasmid
    #233147
    Purpose
    Expresses VDE, PsbS, ZEP from A.thaliana
    Depositor
    Krishna Niyogi
    Article
    Kromdijk et al Science. 2016 Nov 18;354(6314):857-861.
    Inserts
    Resistance to herbicide bialaphos
    Zeaxanthin epoxidase (ABA1 Mustard Weed)
    Photosystem II subunit S (NPQ4 Mustard Weed)
    Violoxanthin de-epoxidase (NPQ1 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    FBA-2, GAPA-1, Rbcs1a, and mas promoter
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233147-plasmid-map-sequence-id-462963

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  18. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

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  19. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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Showing: 321 - 340 of 9378 results