We narrowed to 18,622 results for: Met;
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Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralPromoterCaMKIIaAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PMKO-shNC
Plasmid#64863PurposeExpresses a scrambled shRNADepositorInsertScrambled shRNA
UseRetroviralPromoteru6Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-bArrestin2-TevC-P2A-TdTomato
Plasmid#89869Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronicallyDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseSynthetic BiologyExpressionMammalianMutationR173H in TdTomato (please see depositor comments …PromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-EGFP
Plasmid#65264Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
TagstdTomato-v5-GCaMP6fExpressionMammalianPromoterCMV Immediate EarlyAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM047 [T7-assisted CROPseq sgRNA expression vector]
Plasmid#224899PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNADepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
p15_attB(bxb)_lox
Plasmid#183764PurposeBxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-FLEX-eNpHR-EYFP
Plasmid#89876PurposeTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoterDepositorInserteNpHR=EYFP
UseAAVTagsEYFPExpressionMammalianPromoterTetOAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex-GFP
Plasmid#197883PurposeCan be used to generate AAV virus that will express GFP in the presence of CreDepositorInsertGFP
UseAAVMutationN/APromoterCAGAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Lifeact-7
Plasmid#54663PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorInsertLifeact-mCardinal
ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP FLAG
Plasmid#59760Purposeencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_FLAG
ExpressionMammalianPromoterCAGAvailable SinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168PurposeCodon optimized Cas9_SV40 NLS with intronDepositorInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available SinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only