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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,680 results for: sequence

Showing: 3381 - 3400 of 12680 results

  1. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    G. gallus U6.3
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  2. pJK407

    Plasmid
    #72421
    Purpose
    Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    lipase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72421-plasmid-map-sequence-id-134675

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  3. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  4. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  5. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Promoter
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    16214-plasmid-map-sequence-id-248256

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  7. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-473831

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  8. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  9. pAd/CMV/PBX1-IRES-nEBFP2

    Plasmid
    #29755
    Depositor
    Edward McCabe
    Insert
    Pre-B-cell leukemia transcription factor 1 (PBX1 Human)
    Use
    Tags
    EBFP2, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; EBFP2 is nuclear localized; PBX1 an…
    Promoter
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

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  11. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

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  12. pOX + hSlo2.2

    Plasmid
    #16215
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mutation
    This construct lacks the first 53aa when compared…
    Promoter
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only

  13. pAV hH1-ARRET

    Plasmid
    #111374
    Purpose
    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET w/o polyA
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    hH1
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    111374-plasmid-map-sequence-id-209217

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  14. pENTR hH1-ARRET

    Plasmid
    #111382
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator
    Depositor
    Klaus Holzmann
    Insert
    hH1-ARRET
    Use
    Gateway entry cloning
    Tags
    Expression
    Mutation
    Promoter
    hH1
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111382-plasmid-map-sequence-id-209222

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  15. PPI63

    Plasmid
    #25197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ppi63.0007.0179 (NKTR Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

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  17. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

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  18. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

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  19. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

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  20. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

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Showing: 3381 - 3400 of 12680 results