We narrowed to 3,866 results for: MAK

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mCherry-Cb5-IRES-puromycin-pLVx-EF1a

Plasmid

#177434

Purpose

To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

Depositor

David Andrews

Insert

Cb5 tail anchor (Cyb5r4 Rat)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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modRNAc0-p53DD

Plasmid

#176902

Purpose

IVT template plasmid for making p53DD modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

p53DD

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pB-CAG-TEVp-IRES-mCherry

Plasmid

#174377

Purpose

For making TEVP cell line with hypBase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEV Protease

Expression

Mammalian

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAID 1.1 N-T2A-Bsr

Plasmid

#105985

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAID 1.1C-T2A-Bsr

Plasmid

#105986

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9

Plasmid

#170180

Purpose

IVT template plasmid for making modified Cas9 RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ133C2.0

Plasmid

#99893

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ131C2.0

Plasmid

#99886

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132C2.0

Plasmid

#99889

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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