We narrowed to 4,886 results for: phy

1. pEGFP-CD86-mApple

   Plasmid

   #133860

   Purpose

   Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   CD86 (CD86 Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-G protein Gamma 5

   Plasmid

   #42200

   Depositor

   Narasimhan Gautam

   Article

   Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

   Insert

   Gamma 5 (Gng5 Rat)
   

   Use

   Tags

   Venus(1-155)

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pPKm-293

   Plasmid

   #105817

   Purpose

   pcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTAD

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   TET DBD and MTAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPKm- 226

   Plasmid

   #90497

   Purpose

   pcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PIF3-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN-NG1Tt

   Plasmid

   #44523

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1 (wt) promoter (GAL1 Budding Yeast)
   tetR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGal1 (wt)

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. phumanCas9 (GB0575)

   Plasmid

   #68206

   Purpose

   Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Xl-VSP1 R152Q GFP

   Plasmid

   #51884

   Purpose

   expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTNos:BASTA:PNos (GB0023)

   Plasmid

   #68211

   Purpose

   Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   bar gene
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   PNos

   Available since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AEB-No-aptamer-controlluciferase

   Plasmid

   #63853

   Purpose

   This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   No-aptamer control mRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tac

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPK-351

    Plasmid

    #157921

    Purpose

    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    HA tag and SV40NLS

    Expression

    Mammalian

    Mutation

    pif3 1-523

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EPHB4-gRNA1/2

    Plasmid

    #139974

    Purpose

    gRNA used for knockin EGFP into EPHB4 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EPHB4 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFNB2-gRNA3/4

    Plasmid

    #139973

    Purpose

    gRNA used for knockin tdTomado into EFNB2 allele

    Depositor

    James Thomson

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

    Insert

    EFNB2-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZE21-APX(W41F) dimeric

    Plasmid

    #58199

    Purpose

    Bacterial expression of APX W41F under control of TetR

    Depositor

    Alice Ting

    Article

    Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

    Insert

    pea APX W41F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    W41F

    Promoter

    pL(tetO-1)

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLacIBD::SDRX (GB0673)

    Plasmid

    #68183

    Purpose

    Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    LacI Binding Domain fused to SRDX Transcriptional Repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRTCLCrVA009

    Plasmid

    #22842

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    A DNA containing CHLI fragment
    

    Use

    Cotton vigs vector

    Tags

    Expression

    Bacterial

    Mutation

    Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPKm-163

    Plasmid

    #104854

    Purpose

    pcDNA3 - pCMV - PIF3 - GBD, expressing PIF3 and GBD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF3-GBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. sg resistant gamma-tubulin

    Plasmid

    #104433

    Purpose

    For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed t bp28 to c; g bp30 to a; c bp33 to a; …

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL90 - VND7

    Plasmid

    #116035

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    VND7 (VND7 Mustard Weed)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. ROZA-XL YF

    Plasmid

    #64195

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL YF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL tyrosine phosphorylation site mutated to …

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. r-22. pT7-7-SNCA-9Asn

    Plasmid

    #107297

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-9Asn (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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