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Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only
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pUC57-sgRNA expression vector
Plasmid#51132PurposeFor in vitro transcription of sgRNA from the T7 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterT7Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 (pCMV-IRES-GFP version 3)
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianMutationPromoterCMV, lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDE1093
Plasmid#204227PurposeEpisomal yeast plasmid expressing the Ercas12aDepositorInsertEubacterium rectale cas12a (Ercas12a)
UseTagsExpressionYeastMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ210
Plasmid#109329PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selectionDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1P-E4-luciferase
Plasmid#135592PurposeShort form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vectorDepositorInsertShort form promoter of human CYP26A1 gene (E4)
UseTagsExpressionMutationPromoterHuman CYP26A1 promoter (Short Form, E4)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIhh715bp-Luc
Plasmid#53606PurposeReporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterCMV, T7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMTR-4
Plasmid#66591Purposeexpresses EGFP-tagged MTR-4 in Drosophila cellsDepositorInsertMTR-4 (l(2)35Df Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterActin 5CAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-P
Plasmid#69661PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR mutH(E56A)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-U
Plasmid#69699PurposeUntargetted Bacterial MutagenesisDepositorInsertRBS+ dnaQ926 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-T6
Plasmid#69698PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi AID(Mut7.3.2)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-R6
Plasmid#69689PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 (opt)
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-P9
Plasmid#69668PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi APOBEC1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only