We narrowed to 11,118 results for: ARIA

1. pV1329

   Plasmid

   #111438

   Purpose

   Solo vector pV1326 +sgCgADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgCgADE2
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3s-Cas9-HF1

   Plasmid

   #104173

   Purpose

   Cas9-HF1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   Cas9-HF1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. POM42-mTFP1

   Plasmid

   #69852

   Purpose

   N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1 minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3s-eCas9-1.1

   Plasmid

   #104172

   Purpose

   eCas9-1.1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   eCas9-1.1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-xCas9

   Plasmid

   #140568

   Purpose

   Expresses xCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   A262T R324L S409I E480K E543D M694I E1219V

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pActinin-TagRFP675-N1

   Plasmid

   #44278

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Use

   Tags

   Actinin

   Expression

   Mammalian

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   CMV

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MS2_SC2_S_Omicron

   Plasmid

   #180229

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Omicron (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Jan. 28, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MS2_SC2_S_WT

   Plasmid

   #175044

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pBS7

    Plasmid

    #83774

    Purpose

    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Venus
    

    Use

    Tags

    Venus

    Expression

    Yeast

    Mutation

    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6-mTFP1-His3MX6

    Plasmid

    #69850

    Purpose

    C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PonA-BI-Gl-WT-18xPP7-WT-24xMS2 (Switch)

    Plasmid

    #189793

    Purpose

    Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (Hbb Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-FLAG-Sestrin2-E451A

    Plasmid

    #73677

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pBS-SLBP

    Plasmid

    #45082

    Depositor

    Phillip Newmark

    Article

    Rouhana et al Development. 2014 Jul;141(13):2592-601.

    Insert

    Stem Loop Binding Protein
    

    Use

    RNAi; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 sense, T3 antisense

    Available since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAIO-AsCpf1

    Plasmid

    #137846

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1-NLS-FLAG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-FLAG-Sestrin2-S190W

    Plasmid

    #73670

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S190W changed serine 190 to tryptophan

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-oChEF-citrine

    Plasmid

    #50975

    Purpose

    AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P1-OPNa-delta S

    Plasmid

    #107983

    Purpose

    bacterial expression of full-length OPN

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    OPNa 1080bp-17aa signal (SPP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-TRE3G-NODAL-matMYC

    Plasmid

    #115639

    Purpose

    For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTagRFP675-actin-C1

    Plasmid

    #44277

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    CMV

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTEF-Sup35C

    Plasmid

    #1202

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    Sup35 C-terminus (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Sup35 C-terminus ~254aa-end

    Promoter

    TEF

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mApple-VASP-5

    Plasmid

    #54958

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    VASP
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

24. hSgk1_v3 Flag

    Plasmid

    #86039

    Purpose

    full length human Sgk1_i3 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

    Insert

    Alternate transcript v3 of human Sgk1 (SGK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    v3; S313G

    Promoter

    CMV

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-s34T

    Plasmid

    #28256

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain H3-H4 with Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between H3 and H4

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pNMG

    Plasmid

    #1203

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    NM region of Sup35 (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NM region (1-253aa) of Sup35

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDH6

    Plasmid

    #83777

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y mutations added.

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRDA_381

    Plasmid

    #179092

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    IDT-Cas9_v2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRDA_157

    Plasmid

    #179090

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRDA_156

    Plasmid

    #179089

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge