We narrowed to 7,519 results for: Lif

1. pJET-63-SFFV-T2A-BlaR-polyA-R11a

   Plasmid

   #179894

   Purpose

   Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

   Plasmid

   #179902

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   antiGFPnanobody-mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-61-SFFV-T2A-PuroR-polyA-R11a

   Plasmid

   #179892

   Purpose

   Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

   Plasmid

   #180823

   Purpose

   Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SspB-mCherry-DHPH-ITSN1

   Plasmid

   #176103

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1Donor_G315T_ADRB2_LucReporter

   Plasmid

   #129793

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   G315T

   Promoter

   N/A

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1Donor_W99A_ADRB2_LucReporter

   Plasmid

   #129789

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   W99A

   Promoter

   N/A

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1Donor_N51L_ADRB2_LucReporter

   Plasmid

   #129790

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   N51L

   Promoter

   N/A

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170997

   Purpose

   HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Venus-Bad-2A-pEGFP-C1

    Plasmid

    #166748

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bad-2A (BAD Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SC27 pComb3xss anti-SAPAP2 nanobody

    Plasmid

    #145819

    Purpose

    Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-SAPAP2 nanobody (Rat) recombinant llama nanobody SC27 (Dlgap2 L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-Kv1.5 K+ channel [K7/45R]

    Plasmid

    #140069

    Purpose

    Mammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-DNMT3L [N117/9.5R]

    Plasmid

    #114514

    Purpose

    Mammalian Expression Plasmid of anti-DNMT3L (Human). Derived from hybridoma N117/9.5.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-DNMT3L (Homo sapiens) recombinant mouse monoclonal antibody (DNMT3L Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-TRIP8b (exon 1a/5) [N291C/22R]

    Plasmid

    #114563

    Purpose

    Mammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1+FLAG-KrascomG12D

    Plasmid

    #206844

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-ERBB2-V5/HIS

    Plasmid

    #201103

    Purpose

    expression of human ERBB2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid

    #180437

    Purpose

    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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