We narrowed to 9,393 results for: Coli

1. pSPVpr-hp

   Plasmid

   #48119

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   signal peptide of Vpr
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pANY1

   Plasmid

   #110949

   Purpose

   Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

   Depositor

   Yingfeng An

   Article

   Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

   Insert

   ccd B cassette
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSM15

   Plasmid

   #101640

   Purpose

   Encodes the fluorescent protein EG-4 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   EG-4
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-DEST42-ARL3

   Plasmid

   #67418

   Purpose

   Expression of hArl3 in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL3 (ARL3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET21a - NDH

   Plasmid

   #89626

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   NAD(P)-dependent oxidoreductase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETb-rsGreen0.8

   Plasmid

   #78177

   Purpose

   Expresses rsGreen0.8 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen0.8
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28(a)-p2c-Cas9

   Plasmid

   #242759

   Purpose

   Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

   Depositor

   Jason Rasgon

   Article

   Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

   Insert

   P2C-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1623

   Plasmid

   #239833

   Purpose

   WT OrufIscB E. coli expression for recombinant purification

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB
   

   Use

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-NFMtail

   Plasmid

   #232056

   Purpose

   Expresses construct for surface tethering: R. norvegicus NFM tail domain

   Depositor

   Sanjay Kumar

   Article

   Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

   Insert

   Neurofilament-Medium tail domain
   

   Use

   Tags

   6xHis, thrombin

   Expression

   Bacterial

   Mutation

   cDNA codon-optimized for E. coli; added N-termina…

   Promoter

   T7

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-OuIAD(E502Q)

    Plasmid

    #226973

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(E502Q)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    E502Q

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJExpress401-KDAC8

    Plasmid

    #224208

    Purpose

    Expresses human KDAC8 (HDAC8) in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 6, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLei-sfGFP-D134*

    Plasmid

    #191110

    Purpose

    An E. coli sfGFP reporter with one TAG at position 134

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing D134 to TAG

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLei-sfGFP-V2*D134*

    Plasmid

    #191111

    Purpose

    An E. coli sfGFP reporter with two TAG at positions 2 & 134

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing V2 & D134 to TAG

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-CsHPAD-AE

    Plasmid

    #226982

    Purpose

    Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    CsHPAD-AE
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-CsHPAD_beta-gamma

    Plasmid

    #226981

    Purpose

    Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    CsHPAD_beta-gamma
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-OuIAD(W392A)

    Plasmid

    #226980

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-OuIAD(W392F)

    Plasmid

    #226979

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392F)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392F

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-OuIAD(F401A)

    Plasmid

    #226978

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(F401A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    F401A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-OuIAD(L616E)

    Plasmid

    #226977

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(L616E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    L616E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-OuIAD(R226K)

    Plasmid

    #226976

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226K)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226K

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-OuIAD(R226M)

    Plasmid

    #226975

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226M)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226M

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-OuIAD(R226E)

    Plasmid

    #226974

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-OuIAD(H514E)

    Plasmid

    #226972

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-OuIAD(H514A)

    Plasmid

    #226971

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-OuIAD(C500S)

    Plasmid

    #226970

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(C500S)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    C500S

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-OuIAD(G853A)

    Plasmid

    #226969

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(G853A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    G853A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-OuIAD-AE

    Plasmid

    #226968

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD-AE
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET24a_SMOX

    Plasmid

    #186345

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJB65

    Plasmid

    #226319

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (VTG to AAA MR1-6)-strep
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJB55

    Plasmid

    #226310

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Use

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a- mCherry-FIVAR-HIS-ybbR

    Plasmid

    #169138

    Purpose

    E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    mCherry
    

    Use

    Tags

    6x Histag, FIVAR tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSET-Cm-CspB

    Plasmid

    #215400

    Purpose

    Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspB
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSET-Cm-CspA2

    Plasmid

    #215399

    Purpose

    Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA2
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYPQ121-Split-TALED

    Plasmid

    #218171

    Purpose

    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    

    Use

    Plastid base editing

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSD113A

    Plasmid

    #218133

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Cut190*_pET21b

    Plasmid

    #215404

    Purpose

    Expression construct for Cut190* gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Cut190*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S226P/R228S

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BcC _pET29b

    Plasmid

    #215403

    Purpose

    Expression construct for BcC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    BcC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OoH_pET29b

    Plasmid

    #215424

    Purpose

    Expression construct for OoH gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    OoH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Ph-NylC_pET28a

    Plasmid

    #215425

    Purpose

    Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Ph-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge