Skip to main content
Addgene

We narrowed to 52,908 results for: plasmid

Showing: 8601 - 8650 of 52908 results
  1. pREP81xN_ccdB

    Plasmid
    #79025
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. SRS-MTBD(26/19) AB, ux

    Plasmid
    #22386
    Depositor
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Use
    Tags
    myc
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
  3. A5TSR

    Plasmid
    #50358
    Purpose
    expresses T.gondii MIC2(67-593) domains in mammalian cells
    Depositor
    Insert
    MIC2-A5TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. cmv 16 E7 del EDE

    Plasmid
    #13690
    Depositor
    Insert
    HPV 16 E7 del EDE (E7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    del aa 35-37
    Promoter
    Available since
    March 5, 2007
    Availability
    Academic Institutions and Nonprofits only
  5. cmv 16 E7 E46A

    Plasmid
    #13693
    Depositor
    Insert
    HPV 16 E7 E46A (E7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    aa 46 changed from E to A
    Promoter
    Available since
    March 5, 2007
    Availability
    Academic Institutions and Nonprofits only
  6. cmv 16 E7 N245

    Plasmid
    #13695
    Depositor
    Insert
    HPV 16 E7 N245 (E7 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H2G, D4G, T5M mutations
    Promoter
    Available since
    March 5, 2007
    Availability
    Academic Institutions and Nonprofits only
  7. IRE1bpBS

    Plasmid
    #21878
    Depositor
    Insert
    IRE1b (Ern2 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
  8. SRS-MTBD(19/19), dimer, no myc, ux

    Plasmid
    #22388
    Depositor
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
  9. T4 Lysozyme S117A N132I WT* (says 119)

    Plasmid
    #18527
    Depositor
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S117A N132I WT* (says 119)
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1/GS-DJ1-C53A

    Plasmid
    #29410
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Tags
    V5 epitope
    Expression
    Mammalian
    Mutation
    Cys53Ala
    Promoter
    Available since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only
  11. pRECMYC-Eco2

    Plasmid
    #240706
    Purpose
    pLAM12 vector backbone containing Eco2 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Eco2 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. Sc FLAG-Rev1-pRS425/GAL

    Plasmid
    #241979
    Purpose
    Over-express Sc FLAG-Rev1 in yeast (episomal)
    Depositor
    Insert
    FLAG-Rev1 (REV1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pRECMYC-Tsp1

    Plasmid
    #240712
    Purpose
    pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Tsp11 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pRECMYC-Kva1

    Plasmid
    #240709
    Purpose
    pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Kva1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pRECMYC-Hcl1

    Plasmid
    #240714
    Purpose
    pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Hcl1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pRECMYC-Eas1

    Plasmid
    #240710
    Purpose
    pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Eas1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. pRECMYC-Csp1

    Plasmid
    #240711
    Purpose
    pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Csp1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. pRECMYC-Abe1

    Plasmid
    #240708
    Purpose
    pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Abe1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. pRECMYC-Ban1

    Plasmid
    #240707
    Purpose
    pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Insert
    Ban1 RT, Eco1 ncRNA, MspRecT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. Sc FLAG-S6-rad24-pRSFDuet

    Plasmid
    #240734
    Purpose
    Over-express FLAG-S6-rad24 in E. coli
    Depositor
    Insert
    FLAG-S6-rad24 (RAD24 Budding Yeast)
    Use
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. Sc [ddc1 + rad17]-pET

    Plasmid
    #240740
    Purpose
    Over-express Sc ddc1 & Sc rad17 in E. coli
    Depositor
    Inserts
    ddc1 (DDC1 Budding Yeast)
    rad17 (RAD17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. Sc dpb11-FLAG-pRS405/GAL

    Plasmid
    #240739
    Purpose
    Over-express Sc dpb11-FLAG in Yeast (integrated)
    Depositor
    Insert
    dpb11-FLAG (DPB11 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. Sc FLAG-rad24-pRS404/GAL-L

    Plasmid
    #240735
    Purpose
    Over-express FLAG-rad24 in yeast (integrated)
    Depositor
    Insert
    FLAG-rad24 (RAD24 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  24. Ec (gamma-only)-pET(11a)(ACH–)

    Plasmid
    #241265
    Purpose
    Over-express E. coli Pol III subunit gamma-only in E. coli
    Depositor
    Insert
    dnaXZ (gamma-only)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    No expression of tau
    Promoter
    Available since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. Sc FLAG-POL4-pRS404/GAL-L

    Plasmid
    #241261
    Purpose
    Over-express Sc FLAG-POL4 in yeast (integrated)
    Depositor
    Insert
    FLAG-POL4 (POL4 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. pSIN4-DDX3X-IDR1+DDX6-ΔIDR

    Plasmid
    #240013
    Purpose
    Expresses human DDX6-DDX3X-IDR1+DDX6-ΔIDR fused to 3X Flag in mammalian cells
    Depositor
    Insert
    DDX3X-IDR1+DDX6-ΔIDR
    Use
    Lentiviral
    Tags
    3X Flag
    Expression
    Mutation
    TTAAAAAAAGATAACATC
    Promoter
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pVL1710

    Plasmid
    #239543
    Purpose
    Express Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    Insert
    ScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  28. pVL1708

    Plasmid
    #239542
    Purpose
    Express Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    Insert
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. Sc FLAG-Rev1-pRS404/GAL

    Plasmid
    #241980
    Purpose
    Over-express Sc FLAG-Rev1 in yeast (integrated)
    Depositor
    Insert
    FLAG-Rev1 (REV1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  30. pFA6a-pTagRFP-T-kanMX6

    Plasmid
    #240555
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with TagRFP-T, codon-optimized for S. pombe
    Depositor
    Insert
    pTagRFP-T
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
  31. pLenti-0.5Syn-7 MInGFP-WPRE-pA

    Plasmid
    #233061
    Purpose
    To express 7 Min GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.
    Depositor
    Insert
    7 Min GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  32. pLenti-0.5Syn-Exon 1 of BNDF-GFP-WPRE

    Plasmid
    #233059
    Purpose
    To express GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.
    Depositor
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  33. pAAVS-sA-NeoR

    Plasmid
    #240383
    Purpose
    For insertion into AAV Safe Harbor site, provides G418 resistance
    Depositor
    Insert
    NeoR (neoR )
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    None. Driven by PPP1R12C promoter after homologo…
    Available since
    July 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  34. pMH_AtCK2alpha1_76-409_K318A_H347A_K350A_HC

    Plasmid
    #238514
    Purpose
    Bacterial expression of A. thaliana CK2alpha1 76-409_K318A_H347A_K350A_HC
    Depositor
    Insert
    AtCK2alpha1 (CKA1 Mustard Weed)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K318A_H347A_K350A
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  35. pMH_AtCK2alpha1_76-409_K139M_D249N_HC

    Plasmid
    #238513
    Purpose
    Bacterial expression of A. thaliana CK2alpha1 76-409_K139M_D249N_HC
    Depositor
    Insert
    AtCK2alpha1 (CKA1 Mustard Weed)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K139M_D249N
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  36. pKS1612

    Plasmid
    #239530
    Purpose
    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    Insert
    CpPT1(52-408)-SKL
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  37. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  38. Grx1-roGFP2-PSPC1

    Plasmid
    #236549
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in paraspeckles.
    Insert
    Grx1-roGFP2-PSPC1 (PSPC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  39. pNanog-Halo-Ty2-BactBsd

    Plasmid
    #235511
    Purpose
    C-term HALO targeting construct
    Depositor
    Insert
    Nanog (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    HALO, 2xTy
    Expression
    Mutation
    None
    Promoter
    beta actin
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  40. Sc Rad27-S6- pET24

    Plasmid
    #239205
    Purpose
    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli
    Depositor
    Insert
    Rad27 (RAD27 Budding Yeast)
    Use
    Tags
    S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  41. Sc FLAG-RFC1-pRSFDuet

    Plasmid
    #239201
    Purpose
    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli
    Depositor
    Insert
    RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  42. pTcTurboID-GW_GFP

    Plasmid
    #234715
    Purpose
    Used to express GFP fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling cytoplasmatic spatial control. CDS can be cloned in frame to GFP using EcoRV.
    Depositor
    Insert
    GFP
    Use
    Trypanosomatid expression
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    T7
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  43. pGGSG-Bromo-T7-P2a-Neo

    Plasmid
    #235516
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Neo. Clone into ZraI and AfeI
    Depositor
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, neo
    Expression
    Mutation
    None
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  44. 8061-E02

    Plasmid
    #234240
    Purpose
    Gateway ORF Entry clone of human TGFB2 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    TGFB2 (TGFB2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  45. 8061-E06

    Plasmid
    #234243
    Purpose
    Gateway ORF Entry clone of human TGFBR3 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    TGFBR3 (TGFBR3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  46. 8061-E10

    Plasmid
    #234247
    Purpose
    Gateway ORF Entry clone of human SMAD4 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    SMAD4 (SMAD4 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  47. 8061-E26

    Plasmid
    #234263
    Purpose
    Gateway ORF Entry clone of mouse Tgfb2 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    Tgfb2 (Tgfb2 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  48. 8061-E27

    Plasmid
    #234264
    Purpose
    Gateway ORF Entry clone of mouse Tgfb3 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    Tgfb3 (Tgfb3 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  49. 8061-E30

    Plasmid
    #234267
    Purpose
    Gateway ORF Entry clone of mouse Tgfbr3 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    Tgfbr3 (Tgfbr3 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 8601 - 8650 of 52908 results