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pHAGE-CDK4

Plasmid

#116724

Purpose

Lentiviral expression of CDK4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Publication

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Gateway Cloning

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCM62

Plasmid

#45826

Depositor

Mary Lidstrom

Publication

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Broad host range cloning vector

Promoter

lac

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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dCas-hHDAC3-R265P

Plasmid

#103786

Purpose

Expresses dCas9 fused to human HDAC3 with R265P mutation

Depositor

Zhaolan Zhou

Publication

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed arginine 265 to proline

Promoter

EF1A

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pOPINS-UBE3C

Plasmid

#66711

Purpose

Expresses UBE3C HECT domain in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KiAA0010 (UBE3C )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pENTR-flbio

Plasmid

#30216

Depositor

William Pu

Publication

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

No gene

Use

Gateway Cloning

Tags

Bio and Flag

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Publication

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Expression

Bacterial

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMJH46

Plasmid

#67272

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Publication

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

traJ, cat, oriT

Use

Red recombinase

Promoter

cat gene promoter from PGNS-BAC

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

Plasmid

#163503

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

MinRho

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1, 5F-L YFP

Plasmid

#84913

Purpose

Mammalian expresion of TDP-43 NLS1, 5F-L YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRE145

Plasmid

#118497

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPING

Plasmid

#26046

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

SS peptide-LacZ-KHHHHHH

Tags

KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

Expression

Bacterial, Insect, and Mamm…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBUE411-GGB

Plasmid

#198233

Purpose

Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Publication

Chen et al bioRxiv 2022.09.02.506370

Inserts

GRF4-GIF1 chimera cassette
pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

Ubiquitin and maize PLTP

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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