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pEPYC0CM0522

Plasmid

#196158

Purpose

Phytobricks Level 0 part: MinSyn_305

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_305

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0520

Plasmid

#196157

Purpose

Phytobricks Level 0 part: MinSyn_303

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_303

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0519

Plasmid

#196156

Purpose

Phytobricks Level 0 part: MinSyn_302

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_302

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0515

Plasmid

#196155

Purpose

Phytobricks Level 0 part: MinSyn_301

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_301

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0528

Plasmid

#196160

Purpose

Phytobricks Level 0 part: MinSyn_310

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_310

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0513

Plasmid

#196159

Purpose

Phytobricks Level 0 part: MinSyn_308

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_308

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0529

Plasmid

#196161

Purpose

Phytobricks Level 0 part: MinSyn_311

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

MinSyn_311

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_37_pKD46.10

Plasmid

#178927

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EcAvs1

Plasmid

#188787

Purpose

Avs1 from Escherichia coli NCTC9036

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs1

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAB166

Plasmid

#101668

Purpose

Opto-T7RNAP*(563-F2), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB171

Plasmid

#101670

Purpose

Opto-T7RNAP*(69), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SeAvs1

Plasmid

#188786

Purpose

Avs1 from Salmonella enterica NCTC10718

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs1

Expression

Bacterial

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fCLIP-GS

Plasmid

#188939

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-GS

Plasmid

#188938

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-GS

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-URA

Plasmid

#188927

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Violet

Plasmid

#183139

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Violet

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS713

Plasmid

#188885

Purpose

Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS715

Plasmid

#188887

Purpose

Multisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos Arm

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Ec3Avs4

Plasmid

#188798

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec3Avs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CcAvs4

Plasmid

#188797

Purpose

Avs4 from Corallococcus coralloides DSM2259

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

CcAvs4

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Ec2Avs4

Plasmid

#188795

Purpose

Avs4 from Escherichia coli 382_h

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AcAvs3

Plasmid

#188793

Purpose

Avs3 from Arcobacter cryaerophilus M830A

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

AcAvs3

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSecEffector wo TEVp

Plasmid

#172892

Purpose

atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901

Expression

Bacterial

Promoter

nTETO and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pYCTK010

Plasmid

#176714

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

mfalpha1Ka

Use

Synthetic Biology

Mutation

Codon-optimized for expression in S. cerevisiae

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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