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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Gateway Cloning

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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p2R3a-Tq2CFP-OcsT

Plasmid

#71268

Purpose

Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Tq2CFP

Use

Gateway Cloning

Tags

4xGly linker and octaline synthase terminator

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-H2B-6

Plasmid

#55056

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Publication

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

H2B (H2BC11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Pet22b-mEGFP

Plasmid

#166439

Purpose

Bacterial expression of 6x His tagged mEGFP

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP

Expression

Bacterial

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10-GW

Plasmid

#209370

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoter

Depositor

Lucia Strader

Publication

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Publication

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Bovine, Rat, Human, Mouse)

Tags

Nt: histag-3C protease site

Expression

Bacterial

Promoter

T7 polymerase

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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minCMV-1xmNG-dPCP

Plasmid

#231092

Purpose

Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Publication

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Amber C1

Plasmid

#27797

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mAmber C1

Expression

Mammalian

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-ClfB_N2N3-HIS-ybbr

Plasmid

#101717

Purpose

ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Publication

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ClfB_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1A

Plasmid

#202720

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1A (PIP5K1A Human)

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PBCAG-Cerulean

Plasmid

#40997

Depositor

Joseph Loturco

Publication

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

Cerulean

Expression

Mammalian

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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PBCAG-eGFPt2amRFP

Plasmid

#41001

Depositor

Joseph Loturco

Publication

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

GFPt2a

Expression

Mammalian

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pIRESN2 H2B-GFP-uvr8at(2x)

Plasmid

#44970

Depositor

Thanos Halazonetis

Publication

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

H2B-GFP-UVR8(2x) (UVR8, H2BC21 Mustard Weed, Human)

Tags

internal GFP

Expression

Mammalian

Mutation

tandem repeat of UVR8 (both have Methionine 1 del…

Promoter

pCMV IE

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-mKdm2b (del CxxC domain)-Flag

Plasmid

#61740

Purpose

Express CxxC domain deleted mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Publication

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Flag

Mutation

delete aa 588-624

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSG5-ERRbeta2

Plasmid

#52186

Purpose

Expresses human ERRbeta2 splice variant in mammalian cells

Depositor

Rebecca Riggins

Publication

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHL32 (in cHNL135)

Plasmid

#24387

Depositor

Han Lim

Publication

Lim et al Nat Genet. 2007 Feb . 39(2):269-75.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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