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pAAV-myrSNAP-CON

Plasmid

#60099

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

Depositor

Gregory Jefferis

Article

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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VVV

Plasmid

#27814

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-6-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
MS2-p65-HSF1-P2A-mCherry

Plasmid

#184440

Purpose

MS2 coat protein fused with p65 and hsf1

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

MS2-p65-hsf1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG tubulin GFP

Plasmid

#66105

Purpose

expression of chicken tubulin GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

tubulin

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pRF-QacR

Plasmid

#49369

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

QacR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRF-IcaR

Plasmid

#49362

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTO128_pPZP-NATca

Plasmid

#171105

Purpose

A. tumefaciens TI plasmid encoding NATca in T-DNA

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

NAT (Nourseothricin Resistance)

Use

A. tumefaciens ti plasmid

Promoter

AgTEF2

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET-MAT2A

Plasmid

#164820

Purpose

Expresses MAT2A in E. coli BL21(DE3)

Depositor

Vern Schramm

Article

Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

Insert

Methionine Adenosyltransferase 2A (MAT2A Human)

Tags

6x His Tag

Expression

Bacterial

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_Keima

Plasmid

#127139

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2_Keima (RPS2 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-LIMK2

Plasmid

#23834

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LIMK2 (LIMK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DCK

Plasmid

#23381

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DCK (DCK Human)

Use

Gateway Cloning

Mutation

C57T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK1G1

Plasmid

#23737

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1G1 (CSNK1G1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Expression

Worm

Promoter

cdk-1

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-CDK5

Plasmid

#23699

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK5 (CDK5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ACVR2A

Plasmid

#23654

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR2A (ACVR2A Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-GRAB_HA1m

Plasmid

#190474

Purpose

Express the medium-affinity histamine sensor GRAB_HA1m in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine sensor GRAB_HA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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