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pAAV_CAG(del)-FLEx(loxP)-mCherry(nuclear)-2A-N2cG

Plasmid

#248663

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear mCherry and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-mCherry

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEF_PE2

Plasmid

#199267

Purpose

Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Expression

Mammalian

Mutation

Δ40-903

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-iPEmax-Hygro

Plasmid

#214020

Purpose

inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PEmax

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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T7-VEE-mCherry

Plasmid

#193355

Purpose

Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4

Plasmid

#98946

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161Y

Plasmid

#98951

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161Y: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CXCR4-W161H

Plasmid

#98952

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

W161H: enhanced CXCL12 binding and signaling

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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elavl3:hArr-TEV;he1.1:YFP

Plasmid

#218855

Purpose

D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBI121::chitGFP5:AQ

Plasmid

#240450

Purpose

Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. See Resource Information.

Depositor

Christoph Plieth

Article

Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

Insert

Fusion of Chitinase signal, smGFP5, and Aequorin

Expression

Bacterial and Plant

Mutation

GFP5 for expression plants (PMID 9122158); Solubl…

Promoter

Cauliflower mosaic virus 35S promoter (GenBank AJ…

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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MCS-3_Endofin_LgBiT

Plasmid

#182574

Purpose

This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

Depositor

Bradley McConnell

Article

Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

Insert

zinc finger FYVE-type (ZFYVE16 Human)

Tags

LgBiT (Large BiT)

Expression

Mammalian

Mutation

FYVE domain Q739-K806

Promoter

Herpes simplex virus (HSV)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRAS-IRES-Puro

Plasmid

#120569

Purpose

Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE1

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

Plasmid

#246430

Purpose

All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

O.gue IscB with dead mutations (D60A, H269A) and delta-TID
mCherry
ωRNA

Tags

SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

Expression

Mammalian

Mutation

changed Aspartic Acid 60 to Alanine, changed Hist…

Promoter

CMV and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

Plasmid

#120563

Purpose

Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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