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PX458-gR2A_ISO

Plasmid

#124808

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

Depositor

Martijn Verdoes, Ferenc Scheeren

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

hSPCas9

Use

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pXRDRE-hyg

Plasmid

#31269

Depositor

Andrew Pierce

Insert

XRDRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pGCGFP-puro

Plasmid

#31266

Depositor

Andrew Pierce

Insert

GCGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pGCGFP-blcd

Plasmid

#31263

Depositor

Andrew Pierce

Insert

GCGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pGCGFP-hyg

Plasmid

#31265

Depositor

Andrew Pierce

Insert

GCGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pXRDRE-puro

Plasmid

#31270

Depositor

Andrew Pierce

Insert

XRDRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pXRDRE-blcd

Plasmid

#31267

Depositor

Andrew Pierce

Insert

XRDRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pGLU_66

Plasmid

#225769

Purpose

This is the native promoter from the catP chloramphenicol resistance cassette.

Depositor

Nili Ostrov

Insert

PcatP_[abr]

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGCGFP-G418

Plasmid

#31264

Depositor

Andrew Pierce

Insert

GCGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pXRDRE-G418

Plasmid

#31268

Depositor

Andrew Pierce

Insert

XRDRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-flox-R4R3

Plasmid

#139517

Purpose

Provides two loxP sequences between gene cassettes in the destination vector

Depositor

Minoru Ko

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pH-STEME-1-esgRNA

Plasmid

#138135

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pH-STEME-NG-esgRNA

Plasmid

#138136

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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FtsZ

Plasmid

#74128

Purpose

Expresses FtsZ without a peptide

Depositor

Lynne Regan

Insert

FtsZ

Use

Tags

C-terminal linker sequence (G G S G S G S S)

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector BbsI

Plasmid

#128433

Purpose

Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

Depositor

Izuho Hatada

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9 NGFR

Plasmid

#155279

Purpose

Lentiviral Cas9 expression with NGFR marker

Depositor

Martin Carroll

Insert

spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 CFP

Plasmid

#155278

Purpose

Lentiviral Cas9 expression with CFP marker

Depositor

Martin Carroll

Insert

spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHairyRed (double 35s removed)

Plasmid

#69710

Purpose

Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modified

Depositor

Brett Ferguson, Peter Gresshoff

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti Cas9/SB100

Plasmid

#155294

Purpose

Lentiviral expression of Cas9 and SB100

Depositor

Martin Carroll

Insert

SB100X-P2A-spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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