We narrowed to 16,465 results for: por

1. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

   Plasmid

   #202023

   Purpose

   Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpA T6A-I48V

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCXLE-dCas9VP192-T2A-EGFP-shP53

   Plasmid

   #69535

   Purpose

   Episomal plasmid encoding dCas9VP192 and p53 shRNA

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-GFP-shp53
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TB:4xHRE_RE:MiniP

   Plasmid

   #178313

   Purpose

   4 copies of the HRE DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   4 copies of the heat shock DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLYS5-SDHB-Flag

   Plasmid

   #50055

   Purpose

   Expression of human SDHB in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   SDHB (SDHB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Use

   Tags

   iRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

   Plasmid

   #214990

   Purpose

   Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (Eea1 Mouse)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCCl-MCS_miniCMV_miRFP703

   Plasmid

   #134985

   Purpose

   The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   miRFP703
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAM552

   Plasmid

   #154131

   Purpose

   Expresses wt rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   wt bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH2.30_Gag-MCP-dpol-T2A-eGFP

   Plasmid

   #205538

   Purpose

   Express HIV Gag-MCP-dpol-T2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   HIV Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pol frameshift sequence recoded

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNC1124

    Plasmid

    #41560

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Plasma membrane-targeted optodPLD

    Plasmid

    #140061

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWXLd-RIEP-CeNLuc

    Plasmid

    #135934

    Purpose

    Expresses the CeNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFPZFN1.4-B2H

    Plasmid

    #26791

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pJAM1.18_PB-SDC4-PGK-Blast

    Plasmid

    #205563

    Purpose

    Express human SDC4

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFPZFN2-B2H

    Plasmid

    #26792

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP-ZFN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. eGFP L202 gRNA

    Plasmid

    #119132

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L202 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKanCMV-mClover3-10aa-H2B

    Plasmid

    #74257

    Purpose

    Mammalian expression of N-terminal fusion of mClover3 to H2B

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    H2B
    

    Use

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107131

    Purpose

    cGAL-N driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107132

    Purpose

    cGAL-N driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107136

    Purpose

    cGAL-C driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans eft-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAD/HisB-Fast-FT

    Plasmid

    #31916

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-PKCb1-mEGFP-N1

    Plasmid

    #112265

    Purpose

    ITRACKbeta/IDOCKSbeta Donor construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c beta (Prkcb Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

    Plasmid

    #205760

    Purpose

    Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    YFP-SLBP(18-126)-IRES-H1-Skylan62A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-ICUE3-NLS

    Plasmid

    #138218

    Purpose

    Nuclear-targeted FRET-based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    ICUE3-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC-GGPPipi

    Plasmid

    #53280

    Purpose

    Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge