We narrowed to 4,442 results for: erf

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pcold-6xHis-HRV3Csite-HsPACT

Plasmid

#41097

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

PACT (PRKRA Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
4xHRE_YB TATA-sfGFP-CMV_dsRed

Plasmid

#117399

Purpose

sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pdCas9

Plasmid

#46569

Purpose

Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9
CRISPR array

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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SpyTag-sfGFP

Plasmid

#184226

Purpose

Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpyTag-sfGFP

Tags

His6, SpyTag, and TEV protease cleavage site

Expression

Bacterial

Promoter

T5

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-FIP3

Plasmid

#74753

Purpose

Expressed sfGFP-tagged FIP3 in E. coli for protein production

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab11 family interacting protein 3 (RAB11FIP3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMSP3545-dCas9Str

Plasmid

#153516

Purpose

Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

dCas9Str

Expression

Bacterial

Promoter

nisA

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAW91.dCas9

Plasmid

#104372

Purpose

Expresses dCas9

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

dCas9

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS8c-ddcpf1-Δ

Plasmid

#153039

Purpose

For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Mutation

E993A

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS8c-ddcpf1-rfp

Plasmid

#153038

Purpose

For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Mutation

E993A

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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