We narrowed to 57,378 results for: plasmid
-
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
Plasmid#50471PurposeGi-coupled hM4D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM4D(Gi)-IRES-mCitrine (CHRM4 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposeChemogenetic activator expression plasmidDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
ExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
1.3xWT HBV-Luciferase
Plasmid#61549PurposeThe 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of CoreDepositorInsert1.3 times of the HBV genome with luciferase within the Core ORF
UseLuciferaseExpressionMammalianMutationSubstitution of HBV sequences with luciferase ORF…Available SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120392PurposepAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGPromoterU6Available SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ENVLPE+
Plasmid#232427PurposeExpression plasmid to produce ENVLPE+ particlesDepositorInsertENVLPE+
ExpressionMammalianPromoter2xCMVAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCVneo-HA-ER-Hoxb8
Plasmid#222291PurposeThe most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.DepositorUseRetroviralTagsHAExpressionMammalianMutationThe estrogen receptor hormone binding domain cont…Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
TagsT2A iRFP670ExpressionMammalianAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEA1-GFP
Plasmid#171993PurposeDelivers all prime editing nickase components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianPromoterCMV for Cas9, U6 for gRNAsAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-spe
Plasmid#122000PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein
Plasmid#117848PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, tsPurple
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway CloningTagsMLSAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM446 (Flag-DAS+4))
Plasmid#108321PurposeOrbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-TagRFP657
Plasmid#96939PurposeRetroviral plasmid containing TagRFP657 for labeling of cellsDepositorInsertTag Red Fluorescent Protein 657
ExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STAT3_WT
Plasmid#82235PurposeGateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCX-HO1-2A-EGFP
Plasmid#74672PurposeMammalian expression of HO1 and EGFPDepositorAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRISPseq-BFP-backbone
Plasmid#85707PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only