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pBiT3.1-C_DCAF1:1038-1400

Plasmid

#211053

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_COPB2:1-597

Plasmid

#211054

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_C9orf72:1-481

Plasmid

#210986

Purpose

Mammalian expression of full-length human C9orf72. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

C9orf72:M1-F481 (C9orf72 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_CDC73:1-531

Plasmid

#210983

Purpose

Mammalian expression of full-length human CDC73. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC73:M1-F531 (CDC73 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_CCT5:1-541

Plasmid

#210979

Purpose

Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CCT5:M1-E541 (CCT5 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_FBXW5:1-566

Plasmid

#210981

Purpose

Mammalian expression of full-length human FBXW5. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW5:M1-R566 (FBXW5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_ACTB:1-375

Plasmid

#210971

Purpose

Mammalian expression of full-length human ACTB. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DCAF12:1-453

Plasmid

#210967

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_DCAF1:1038-1400

Plasmid

#210963

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CORO1A:1-461

Plasmid

#210964

Purpose

Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW11:M1-R542 (FBXW11 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SKIC8:M1-I305 (SKIC8 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CMV500 A-FOS

Plasmid

#33353

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

FOS (Fos Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative of c-Jun; heterodimerizes and i…

Promoter

CMV

Available Since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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CRY2olig(FL)-mCherry

Plasmid

#60033

Purpose

Expresses a fusion of full length CRY2 (E490G) with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G, NLS mutated

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCherry

Plasmid

#60032

Purpose

Expresses a fusion of CRY2PHR E490G with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2PHR E490G (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
E. coli 16S toehold switch sensor

Plasmid

#110698

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E. coli mRNA toehold switch sensor

Plasmid

#110708

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Article

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pR I-Ab alpha BirA

Plasmid

#113638

Purpose

Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab alpha BirA (H2-Ab1 Mouse)

Tags

BirA biotinylation signal site and fused to Fos-J…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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