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pKC50

Plasmid

#226778

Purpose

Expresses Drosophila UNC-104(V6M) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (V6M) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Valine 6 to Methionine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC28

Plasmid

#226776

Purpose

Expresses Drosophila UNC-104(G618R) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (G618R) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

G618R

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mma PylRS

Plasmid

#154762

Purpose

plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-MUT-YFP

Plasmid

#235144

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLAG-HA-T6B-MUT-YFP

Plasmid

#235140

Purpose

Expresses the mutant T6B peptide fused with YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mutant T6B peptide

Use

AAV

Tags

FLAG/HA

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_SKIL

Plasmid

#45289

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SNON (SKIL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBR322-Pdp1_NTD-ZFD_L

Plasmid

#198276

Purpose

For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_L

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_TNIK

Plasmid

#45276

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TNIK (TNIK Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry

Plasmid

#180652

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1(118-1274)

Plasmid

#221207

Purpose

ScChd1-aa118-1274 chromatin remodeler

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

Plasmid

#182825

Purpose

Cre-dependent expression of H2B-APEX fusion

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

H2B.APEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0663 pET45b(+) KraIscB-1 TwinStrep

Plasmid

#176536

Purpose

Bacterial expression of KraIscB-1-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

KraIscB-1

Tags

TwinStrep

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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SB-SRT-Puro

Plasmid

#154888

Purpose

Sleeping Beauty self-reporting transposon with puromycin reporter

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

Sleeping beauty SRT

Use

Transposon

Expression

Mammalian

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Article

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-PLK1-mCherry

Plasmid

#127154

Purpose

Expresses mCherry PLK1 in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1

Insert

PLK1-mCherry (PLK1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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