We narrowed to 13,665 results for: EGF
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Plasmid#235307PurposeLentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)DepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE
Plasmid#235308PurposeLentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)DepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40
Plasmid#233373PurposeEF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transductionDepositorInsertsNES-Kinprola_on-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD
UseLentiviralTagsFLAG, NES, and mEGFPExpressionMutationPromoterEF1αAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40
Plasmid#233372PurposeEF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transductionDepositorInsertsNES-Kinprola_PKA_T/A-mEGFP
FLAG-SV40NLS-Cas9-NLS-T2A-BSD
UseLentiviralTagsFLAG, NES, and mEGFPExpressionMutationPromoterEF1αAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)
Plasmid#223784PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
UseTagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A/W276A/I279A/I307A/V310A; deletion of …PromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC
Plasmid#213535PurposeExpresses the split transcription factors in cultured neuron and mouse brainDepositorInsertSspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
UseAAVTagsEGFP and HAExpressionMammalianMutationPromoterSynapsinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)
Plasmid#176140PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resisDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutations Lys35 to Ala, Lys6…PromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113699PurposeDox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176459PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113700PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176460PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP
Plasmid#230075PurposePartial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterfabp10aAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
Plasmid#210516Purposeexpresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cellsDepositorInsertNTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
Plasmid#210505Purposeexpresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cellsDepositorInsertNTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-DNER
Plasmid#51053Purposeexpression of HA-DNER in mammalian cellsDepositorInsertDNER (Dner Mouse)
UseTagsHA and signal peptideExpressionMammalianMutationwithout endogenous signal peptide (1-221bp)PromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Human SHIP2
Plasmid#124645PurposeExpresses HIS tagged human SHIP2 in COS-7/HEK cellsDepositorInsertINPPL1 (INPPL1 Human)
UseTagsHis Xpress (N-terminal)ExpressionMammalianMutationcoding sequence starts at amino acid 18 as calcul…PromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK2 KD
Plasmid#12183DepositorInsertMEKK2 (Map3k2 Mouse)
UseTagsHAExpressionMammalianMutationKinase dead: K385MPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Src
Plasmid#19965DepositorInsertSrc (SRC Chicken)
UseTagsExpressionMammalianMutationK295MPromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianMutationPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertBetacellulin (BTC Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAR3040-spitz
Plasmid#38341DepositorInsertspitz (spi Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
UseTagsExpressionYeastMutationACT2-SA236PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB215
Plasmid#185068PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)DepositorInsertWAK2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
spitz (9 kb)
Plasmid#38340DepositorInsertspitz (spi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5 c-Fyn
Plasmid#16032DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Fyn
Plasmid#16033DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDominant negative: K299MPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
SCUBE1-bio-His
Plasmid#53415PurposeExpresses full-length Signal peptide, CUB and EGF-like domain-containing protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSCUBE1 (SCUBE1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hAREG
Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPGN
Plasmid#199232PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpithelial mitogen isoform 6 (EPGN Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn deltaSH3
Plasmid#16034DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDelta SH3: deletion of amino acids 76-141PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn SH2:R176E
Plasmid#16035DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationSH2 point mutant: R176EPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-Lact-C2
Plasmid#109553PurposeMultisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertthe C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
psd44-Lactadherin46
Plasmid#46830Purposeexpression of human lactadherin in mammalian cellsDepositorInsertMFGE8 (MFGE8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only