We narrowed to 7,376 results for: ESP

Showing: 3521 - 3540 of 7376 results

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

Plasmid

#214990

Purpose

Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

CRISPR

Tags

3xFLAG

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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Fusion of Dengue2 NS2B-NS3

Plasmid

#204800

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2trLAT-2P

Plasmid

#203748

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAToC27AC30A (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Mutation

C27A, C30A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-NucGFP

Plasmid

#184696

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP1-HA

Plasmid

#183946

Purpose

Expresses human HA-tagged DIPP1 from yeast GPD promoter

Depositor

Michael Downey

Article

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP1 (NUDT3 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

b2AR (ADRB2 Human)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-OEP7(1–50)

Plasmid

#100602

Purpose

Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50)-Citrine (OEP7 Mustard Weed)

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Promoter

-

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsE

Plasmid

#97019

Purpose

Expressing Pseudomonas aeruginosa pqsE protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Quinolone signal response protein

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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R777-E292 Hs.RPS6KA2-nostop

Plasmid

#70576

Purpose

Gateway ORF clone of human RPS6KA2 [NM_021135.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E102 Hs.HRAS-nostop

Plasmid

#70386

Purpose

Gateway ORF clone of human HRAS [NM_005343.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mrpl11-mod

Plasmid

#49569

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

Mrpl11-mod

Use

Luciferase

Mutation

T/A mutation in ATG start site

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
8061-E43

Plasmid

#234280

Purpose

Gateway ORF Entry clone of mouse Bmpr1b with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

Bmpr1b (Bmpr1b Mouse)

Use

Gateway Cloning

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a-HSF1-GFP

Plasmid

#227820

Purpose

Expresses HSF1 fused to GFP from EF1a promoter

Depositor

Jeffrey Gerst

Article

Pataki et al Sci Adv. 2025 Dec 5;11(49):eadu0315. doi

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

3xHA and GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR4_CELF1

Plasmid

#106098

Purpose

Entry vector for CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Gateway Cloning

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETB-roGFP1-R10

Plasmid

#83310

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R10

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/F223R/S202K/K41D

Promoter

T7

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only