We narrowed to 11,278 results for: VARS

1. pGTRm

   Plasmid

   #127197

   Purpose

   pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA PCR template
   

   Use

   Template plasmid for generation of golden gate pc…

   Promoter

   None

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a hMDH2

   Plasmid

   #204230

   Purpose

   Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH2 (MDH2 Human)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRS_8-ChrimsonR-tdTomato

   Plasmid

   #192587

   Purpose

   AAV-vector for optogenetic activation of noradrenergic neurons

   Depositor

   Simon Wiegert

   Article

   Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

   Insert

   ChrimsonR
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   PRSX8

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_3E5E_luciferase

   Plasmid

   #103055

   Purpose

   Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebolavirus minigenome
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFFV_sfCherry3V(1-10)

   Plasmid

   #117483

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3V(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3V(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   6 substitutions (K45R, G52D, L83W, T106A, K182R, …

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Klotho (membrane)

   Plasmid

   #17712

   Depositor

   Hal Dietz

   Article

   Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

   Insert

   Klotho (KL Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   P101S

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

   Plasmid

   #159849

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB076i3

   Plasmid

   #79198

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad-CA TAG126

   Plasmid

   #105836

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 126

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-BICD1

    Plasmid

    #49487

    Purpose

    expresses EGFP-BICD1 in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu

    Insert

    BICD1 (BICD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ** see notes

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEY121

    Plasmid

    #223468

    Purpose

    sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    sra-6 (sra-6 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDB180

    Plasmid

    #80677

    Purpose

    for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

    Depositor

    David Baker

    Article

    Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

    Type

    Empty backbone

    Tags

    OsmY-10His-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-tRFP

    Plasmid

    #230938

    Purpose

    Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

    Depositor

    Hyejung Won

    Article

    Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSFFV-SpyTag-sfCherry2(11)-TagBFP

    Plasmid

    #117485

    Purpose

    Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyTag002-GG-sfCherry2(11)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Expression

    Mammalian

    Promoter

    caggs

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

19. pV1382

    Plasmid

    #111436

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-AC 4-μHOM

    Plasmid

    #113619

    Purpose

    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    4-μHOM variant of EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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