We narrowed to 8,296 results for: construct

1. pFGC5941-PacI-ath-STTM159

   Plasmid

   #84084

   Purpose

   target miRNA159 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM159
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC5941DR5-PacI-ath-STTM390

   Plasmid

   #84117

   Purpose

   target miRNA390 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM390
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p3E-FKBP-HA no-pA

   Plasmid

   #82598

   Purpose

   HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FKBP-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFGC5941-PacI-ath-STTM858a/b

   Plasmid

   #84177

   Purpose

   target miRNA858 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM858a/b
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046789

   Plasmid

   #145660

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. MSCV Puro-EFS:GFP

   Plasmid

   #68484

   Purpose

   Retrovirus expressing GFP driven by EFS promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046921

   Plasmid

   #145755

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGBW-m4136463

   Plasmid

   #149527

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pFGC5941-PacI-ath-STTM828

   Plasmid

   #84157

   Purpose

   target miRNA828 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM828
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC5941-PacI-ath-STTM843

    Plasmid

    #84169

    Purpose

    target miRNA843 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM843
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFGC5941-PacI-ath-STTM845

    Plasmid

    #84170

    Purpose

    target miRNA845 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM845
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Ku70-TC

    Plasmid

    #46955

    Purpose

    Plasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    XRCC6 targeting construct (XRCC6 Human)
    

    Use

    AAV; R

    Tags

    GFP-FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-2Aneo v2

    Plasmid

    #80033

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-2Aneo

    Plasmid

    #80032

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046925

    Plasmid

    #145694

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. p3E-FRB-HA no-pA

    Plasmid

    #82594

    Purpose

    3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFGC5941-PacI-ath-STTM173

    Plasmid

    #84101

    Purpose

    target miRNA173 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM173
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFGC5941-PacI-ath-STTM168

    Plasmid

    #84093

    Purpose

    target miRNA168 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM168
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge