We narrowed to 14,580 results for: pET

1. pET-Duet1_6xHis-TEV-GABARAP-Gly

   Plasmid

   #190930

   Purpose

   Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Ends with Glycine 116

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEW3: pET-32a Dot1L(2-416)

   Plasmid

   #124098

   Purpose

   Dot1L(2-416) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-416) (DOT1L Human)
   

   Use

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-His10-SHP1_PTPase (243-541aa)

   Plasmid

   #180812

   Purpose

   Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SHP1_PTPase (243-541aa) (PTPN6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a+GFP-CAMSAP3 CKK

   Plasmid

   #59033

   Purpose

   expresses CKK domain of CAMSAP3 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CKK domain (CAMSAP3 Mouse)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pET29b-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175783

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-mVenus-dspB(E184Q)-6xHis

   Plasmid

   #176576

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a-His6-SUMO-VAMP3

   Plasmid

   #135552

   Purpose

   Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP3
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETNKI-his-SUMO-RNF168ring (1-189)

   Plasmid

   #63570

   Purpose

   Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   6xHis and SUMO3

   Expression

   Bacterial

   Mutation

   Ring domain only (residues 1-189)

   Promoter

   T7

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-ybbR-HIS-Xyl-DocI

   Plasmid

   #58711

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Xyl-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Threonin 120 to Cysteine in Xylanase Doma…

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Use

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXB8 (murine) HIS-tag pET

    Plasmid

    #8540

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB8 (Hoxb8 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    starts at amino acid #2 (Ser)

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET-45b-RUNX2(WT)-IDR-mCherry

    Plasmid

    #145286

    Purpose

    Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(WT)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-45b-HOXD13-IDR-mCherry

    Plasmid

    #145280

    Purpose

    Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET empty polycistronic destination vector (2Z)

    Plasmid

    #29776

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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