We narrowed to 15,323 results for: pET

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(E167)

Plasmid

#166004

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(E167)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(D165)

Plasmid

#166003

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(D165)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(C164)

Plasmid

#166002

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(C164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(N180)

Plasmid

#166000

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(N180)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(A165)

Plasmid

#165999

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A165)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Tags

6His

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(L166)

Plasmid

#165995

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(L166)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(G164)

Plasmid

#165993

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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CPH1141 (pET151_Spt6 1247-1451)

Plasmid

#91815

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

deleted residues 1-1246

Promoter

T7

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(Y205F)

Plasmid

#155132

Purpose

Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

Y205F, insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE Y165A

Plasmid

#139428

Purpose

GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed tyrosine 165 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE P199A

Plasmid

#139427

Purpose

GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed proline 199 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE I137A

Plasmid

#139319

Purpose

GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed isoleucine 137 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

Plasmid

#136688

Purpose

Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 (BECN1 Human)

Tags

Trigger Factor

Expression

Bacterial

Mutation

DNA optimized for E. coli expression w/ three mut…

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET-29b-3xVSVG-GSK3(1-97)

Plasmid

#129583

Purpose

Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

GSK3a(1-97) (GSK3A Human)

Tags

6xHistidine

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP18-RBS-pET-MV_RN

Plasmid

#127014

Purpose

Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part RBS-pET-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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