164,799 results

1. pLJM60

   Plasmid

   #34610

   Depositor

   David Sabatini

   Insert

   deptor (DEPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-Sox2-IP

   Plasmid

   #13459

   Depositor

   Shinya Yamanaka

   Article

   Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_PEmax-2A-MLH1dn-2A-BSD

   Plasmid

   #234072

   Purpose

   For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax-2A-MLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α core promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-(empty)-TAG

   Plasmid

   #138209

   Purpose

   To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Type

   Empty backbone

   Use

   T7 transcription

   Tags

   TAG sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter, T7 Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   David Schaffer, John Flannery

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   7mer insertion in the AAV2 cap

   Promoter

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXB-EBNA1

    Plasmid

    #41857

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-HA_WT POLH

    Plasmid

    #201671

    Purpose

    Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    Polymerase eta (POLH Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-mCherry

    Plasmid

    #20972

    Purpose

    H2B fused to mCherry for chromatin visualization

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLX-PGK-hTERT

    Plasmid

    #114315

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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