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Showing: 7121 - 7160 of 10228 results

  7. pET21a - MPX

    Plasmid
    #89619
    Purpose
    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Mitocondrial Tryparedoxin peroxidase
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89619-plasmid-map-sequence-id-176691

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  8. pET21a - EtfA

    Plasmid
    #89616
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Electron transport flavoprotein alpha subunit
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89616-plasmid-map-sequence-id-176692

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  9. pET21a - EtfB

    Plasmid
    #89617
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    electron-transfer-flavoprotein, alpha polypeptide
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89617-plasmid-map-sequence-id-176708

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  12. pET151-Vps4(101-437) L151D

    Plasmid
    #87736
    Purpose
    Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Leu 151 to Asp
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87736-plasmid-map-sequence-id-175371

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  20. pJK516

    Plasmid
    #71578
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to glutamine
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    71578-plasmid-map-sequence-id-130937

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  21. pJK524

    Plasmid
    #71580
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes asparagine-347 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71580-plasmid-map-sequence-id-130939

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  22. pJK513

    Plasmid
    #71576
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-294 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71576-plasmid-map-sequence-id-130935

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  27. pJK564

    Plasmid
    #71581
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes serine-71 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71581-plasmid-map-sequence-id-130940

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  28. pJK517

    Plasmid
    #71579
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to aspartate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71579-plasmid-map-sequence-id-130938

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  29. pJK514

    Plasmid
    #71577
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamine-435 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71577-plasmid-map-sequence-id-130936

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  30. pJK512

    Plasmid
    #71575
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes arginine-228 to glutamate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71575-plasmid-map-sequence-id-130934

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  31. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71571-plasmid-map-sequence-id-130928

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  32. pJK385

    Plasmid
    #71572
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71572-plasmid-map-sequence-id-130931

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  33. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

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  34. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

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Showing: 7121 - 7160 of 10228 results