We narrowed to 26,176 results for: vit;

1. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

   Plasmid

   #233065

   Purpose

   Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Leucine 928 to Alanine; Methionine 929 to Alanine

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK-1-BRAF_WT&Mut-SM

   Plasmid

   #215916

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiCHECK-1-BRAF_WT-SM

   Plasmid

   #215917

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiCHECK-1-BRAF_Mut1-SM

   Plasmid

   #215918

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-1-BRAF_Mut2-SM

   Plasmid

   #215919

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psiCHECK-1-BRAF_WT&Mut-CM

   Plasmid

   #215920

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   one complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psiCHECK-1-BRAF_WT-CM

   Plasmid

   #215921

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   one complete-matched (CM) sequence of siBRAF_ WT (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK-1-BRAF_Mut1-CM

   Plasmid

   #215922

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   one complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK-1-BRAF_Mut2-CM

   Plasmid

   #215923

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_V5-TWIST1_NEUROD1L

    Plasmid

    #215043

    Purpose

    Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Left portion of TWIST1 bHLH loop (aa137-139, TLP)…

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_V5-TWIST1_NEUROD1R

    Plasmid

    #215044

    Purpose

    Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1)

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Right portion of TWIST1 bHLH loop (aa139-141, PSD…

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT22gCfpUbm5En2E4PCh(#251)

    Plasmid

    #184083

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT22Ubm5Eng2bE4PCh(#284)

    Plasmid

    #184088

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT22UbHEn2E4PCh(#262)

    Plasmid

    #184084

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22Ubm5Eng2aE4PCh(#283)

    Plasmid

    #184087

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-CARMA2sh-delta458-500

    Plasmid

    #97074

    Purpose

    Expresses mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 458-500

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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