We narrowed to 841 results for: nanobodies

1. pOPINVHH_cys_his

   Plasmid

   #210403

   Purpose

   For expression of VHH with C-terminal cysteine and his tag

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   cys-his6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINVHH_his_flag

   Plasmid

   #210404

   Purpose

   For expression of VHH with C-terminal his and Flag tags

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   his6-flag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOPINVHH_sortase_his

   Plasmid

   #210406

   Purpose

   For expression of VHH with C-terminal sortase and his tags

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   sortase-his6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPINVHH_snap_his

   Plasmid

   #210407

   Purpose

   For expression of VHH with C-terminal snap protein and his tag

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYTSG

   Plasmid

   #159419

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIR-Fb2E7

   Plasmid

   #184681

   Purpose

   NIR-Fb to HIV gp41

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb2E7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFTSG

   Plasmid

   #162389

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNIR-Fb4EIG

   Plasmid

   #184683

   Purpose

   NIR-Fb to E.coli dihydrofolate reductase

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb4EIG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTrcHis-7D12Gly42TAG

   Plasmid

   #125265

   Purpose

   Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 Gly42 nanobody
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   trc promoter

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCOLA-Gent-ppiB

    Plasmid

    #202482

    Purpose

    Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    peptidyl-prolyl cis-trans isomerase (ppiB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM6-Amp-T7-GFPuv

    Plasmid

    #202463

    Purpose

    Expresses GFPuv with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCASCADE-LCAmp-trxB

    Plasmid

    #202465

    Purpose

    Expresses a guide RNA to silence trxB after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    trxB guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ugpB

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAL4-sfGFP(1-157)

    Plasmid

    #220743

    Purpose

    GAL4-sfGFP(1-157) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(1-157)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAL4-sfGFP(158-238)

    Plasmid

    #220744

    Purpose

    GAL4-sfGFP(158-238) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(158-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAL4-NIR-FbLAG30

    Plasmid

    #220746

    Purpose

    GAL4-NIR-FbLAG30 construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIR-FbLAG16

    Plasmid

    #220739

    Purpose

    NIR-FbLAG16 expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG16
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNIR-FbLAG30

    Plasmid

    #220740

    Purpose

    NIR-FbLAG30 expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIR-Fb(718)

    Plasmid

    #220742

    Purpose

    NIR-Fb(718) expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-Fb(718)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINVHH_A8_His

    Plasmid

    #201718

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_A8 (S lama glama (llama))
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOPINVHH_A10-5_His

    Plasmid

    #201720

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_A10-5 (S lama glama (llama))
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOPINVHH_B5-5_His

    Plasmid

    #201721

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH B5-5 (S lama glama (llama))
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Rluc8-NbV5

    Plasmid

    #201479

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-Rluc8
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NbV5-Rluc8

    Plasmid

    #201480

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    Rluc8-NbV5
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-GBP1-10gly-lexADBD

    Plasmid

    #50795

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-lexA DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Use

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

    Plasmid

    #184688

    Purpose

    Mammalian expression, miRFP670nano3

    Depositor

    Axel Nimmerjahn, Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSYN

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LgBit-NbV5

    Plasmid

    #201476

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    LgBit-NbV5
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NbAlfa-GFP2

    Plasmid

    #201485

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbAlfa-GFP2
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge