Addgene

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Rat SERCA2a (pMT2)

Plasmid

#75183

Purpose

Mammalian expression of ATP2A2

Depositor

Gary Shull, Jonathan Lytton

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Rat SERCA2b (pMT2)

Plasmid

#75184

Purpose

Mammalian expression of ATP2A2

Depositor

Gary Shull, Jonathan Lytton

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free PcPTS

Plasmid

#182483

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
PTS

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10 and GAL7

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

Plasmid

#196191

Purpose

Tagging human SOX2 gene with P2A-EGFP at the C-terminus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)

Use

Homology repair donor

Tags

Expression

Mutation

G2913A mutation to replace the guide cutting site…

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

Plasmid

#196196

Purpose

homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)

Use

Homology repair donor

Tags

V5-APEX2-mScarlet-I

Expression

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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CbetaS-pA

Plasmid

#32104

Depositor

Yardena Samuels, Michael Cole

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pOPINA

Plasmid

#41141

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINCDF

Plasmid

#41132

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pTN186

Plasmid

#34890

Depositor

Mitsuyasu Hasebe

Type

Empty backbone

Use

Moss targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only
pOPINB

Plasmid

#41142

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINCDE

Plasmid

#41135

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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JDS88

Plasmid

#40319

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 KKR (+) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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JDS92

Plasmid

#40321

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 ELD (-) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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JDS82

Plasmid

#40317

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 KKR (+) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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JDS94

Plasmid

#40322

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 ELD (-) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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JDS98

Plasmid

#40323

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 ELD (-) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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JDS84

Plasmid

#40318

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 KKR (+) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINRSF

Plasmid

#41128

Depositor

Ray Owens

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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JDS80

Plasmid

#40316

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 KKR (+) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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JDS90

Plasmid

#40320

Depositor

Keith Joung

Type

Empty backbone

Use

Tags

3XFLAG and FOK1 ELD (-) heterodimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge