We narrowed to 2,009 results for: D10

Showing: 341 - 360 of 2009 results

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pGEX6P1‐Afu‐rnhB D101N

Plasmid

#108695

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

D101N catalytic site mutation

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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Top7 D9T D10V N14T

Plasmid

#12473

Depositor

David Baker

Insert

Top7 D9T D10V N14T

Tags

6xHis

Expression

Bacterial

Mutation

D9T D10V N14T

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pPD1028 ZF43x6-Compact(SV40Min)_EYFP

Plasmid

#138912

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(SV40min)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(SV40min)

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD1043 TRE3GV mTagBFP2

Plasmid

#253903

Purpose

Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and mTagBFP2 in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-D10A

Plasmid

#250796

Purpose

Drosophila transgenic vector: Gateway cloning destination vector for expressing enhancer driven Cas9-derived nickase D10A.

Depositor

Chun Han

Article

Shen et al bioRxiv 2025.10.30.685647

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd10 gRNA

Plasmid

#246554

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd10 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd10 gRNA (Fzd10 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Cd109 gRNA

Plasmid

#246571

Purpose

CRISPR vector co-expressing Cas9 and a mouse Cd109 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Cd109 gRNA (Cd109 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101N

Plasmid

#224348

Purpose

Expresses human KDAC8 (HDAC8) D101N in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to asparagine

Promoter

T5

Available Since

Feb. 19, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-parkin D106A

Plasmid

#227362

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin D106A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Salsa-BC238-P3D10

Plasmid

#203079

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC238

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC238

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC142-P2D10

Plasmid

#202983

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC142

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC142

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC46-P1D10

Plasmid

#202887

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC46

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC46

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

Plasmid

#199253

Purpose

Expression construct encoding the high specificity SpCas9-KA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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