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CL20_mEGFP_DDX3X_MRAS

Plasmid

#205803

Purpose

Express mEGFP-tagged fusion protein, DDX3X_MRAS from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DDX3X_MRAS (DDX3X, MRAS Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D

Plasmid

#213597

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAPK1

Plasmid

#116760

Purpose

Lentiviral expression of MAPK1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAPK1-E322K

Plasmid

#116432

Purpose

Lentiviral expression of MAPK1 E322K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

E322K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/D321N

Plasmid

#213598

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, D321N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/Y263A

Plasmid

#213599

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, Y263A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv9.1 K+ channel [N461/19R]

Plasmid

#206592

Purpose

Mammalian Expression Plasmid of anti-Kv9.1 K+ channel (Mouse). Derived from hybridoma N461/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv9.1 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcns1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

missing first codon

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-2b]

Plasmid

#220431

Purpose

Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody. (KCNQ1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R]

Plasmid

#177539

Purpose

Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human). Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-rat IgG2a] - Chimeric

Plasmid

#231840

Purpose

Mammalian expression plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
SHP2(T253M/Q257L)@pLKO-Trex-SBP

Plasmid

#85458

Purpose

SHP2 mutant gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

T253M/Q257L

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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