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We narrowed to 17,849 results for: Mut

Showing: 7161 - 7200 of 17849 results

  10. pCMV(MinDis)-iCyt_BrEt_SnFR_PM

    Plasmid
    #177747
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177747-plasmid-map-sequence-id-349063

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  11. _pCMV(MinDis)-iCyt_F_SnFR_PM

    Plasmid
    #177745
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177745-plasmid-map-sequence-id-349553

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  13. pCMV(MinDis)-iCyt_BrEt_SnFR_ER

    Plasmid
    #177746
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177746-plasmid-map-sequence-id-349555

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  14. _pCMV(MinDis)-iCyt_F_SnFR_ER

    Plasmid
    #177744
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177744-plasmid-map-sequence-id-349548

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  29. pEGFP_∆R13–DD-talin1 (1-2299)

    Plasmid
    #166125
    Purpose
    For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.
    Depositor
    Vesa Hytönen
    Article
    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.
    Insert
    ∆R13–DD-Talin-1 (Tln1 Mouse)
    Use
    Cmv promoter
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    residues 1-2299, deletion of dimerization domain …
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166125-plasmid-map-sequence-id-330294

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  30. pLJM1-Flag-Rheb-12Rs

    Plasmid
    #165023
    Purpose
    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-12Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    All the lysine residues except K19 and K120 are c…
    Promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165023-plasmid-map-sequence-id-326718

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  31. ST-Tstem-GFP

    Plasmid
    #162501
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The Tac stem region is inserted within ST6Gal1
    Promoter
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162501-plasmid-map-sequence-id-323506

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  32. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

    Plasmid
    #154778
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Use
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154778-plasmid-map-sequence-id-310407

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  33. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

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  35. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  36. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  37. AdMKP-3 S159AS197A

    Plasmid
    #131523
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Article
    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    No
    Expression
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131523-plasmid-map-sequence-id-256915

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  38. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid
    #131111
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131111-plasmid-map-sequence-id-257476

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  39. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid
    #131112
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131112-plasmid-map-sequence-id-257485

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  40. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid
    #131113
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131113-plasmid-map-sequence-id-257490

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Showing: 7161 - 7200 of 17849 results