We narrowed to 29,109 results for: PLE;

1. pSBbi-TIR1-tTA/Neo

   Plasmid

   #171684

   Purpose

   constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

   Depositor

   Randy Poon

   Article

   Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

   Insert

   Neomycin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RPBSA promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_D10A-1x3

   Plasmid

   #58773

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_sg83-PDGFR_EF1a_iRFP670

   Plasmid

   #186311

   Purpose

   Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg83-PDGFR
   iRFP670
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_TRE_sg88-PDGFR_EF1a_eBFP2

   Plasmid

   #186312

   Purpose

   Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg88-PDGFR
   eBFP2
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-HDR-sphys-3XHA

   Plasmid

   #163690

   Purpose

   plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

   Depositor

   Bernardo Sabatini

   Article

   Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

   Insert

   triple HA tag insertion into endogenous mouse synaptophysin gene
   

   Use

   AAV

   Tags

   triple HA tag

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG711_lexO-AscI_LexA-TF_HIS3MX

   Plasmid

   #154820

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBDT

   Plasmid

   #62660

   Purpose

   Targeting vector for PITT landing pad knock-in into the Rosa26 locus

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Inserts

   eGFP
   neomycin
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

   Plasmid

   #140087

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBDU

    Plasmid

    #62661

    Purpose

    Targeting vector for PITT landing pad knock-in into the Rosa26 locus

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Inserts

    eGFP
    neomycin
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBGV

    Plasmid

    #62664

    Purpose

    Donor vector for mCFP knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    mCFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR21

    Plasmid

    #69156

    Purpose

    read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p201N 1514

    Plasmid

    #47025

    Purpose

    Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

    Depositor

    Wayne Parrott

    Article

    Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

    Inserts

    gma-miR1514 recognition sequence
    nptII selectable marker
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    GmUbi and StUbi-3

    Available since

    Aug. 30, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. SAGA032

    Plasmid

    #240781

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SAGA031

    Plasmid

    #240780

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SAGA030

    Plasmid

    #240779

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SAGA023

    Plasmid

    #240772

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SAGA022

    Plasmid

    #240771

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SAGA021

    Plasmid

    #240770

    Purpose

    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SAGA019

    Plasmid

    #240768

    Purpose

    Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

    Depositor

    Morten Norholm

    Article

    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

    Insert

    SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutation on translation initiation region of tetA

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge