We narrowed to 34,848 results for: CaS;

1. pdCas9-DNMT3A-PuroR_hNT

   Plasmid

   #71830

   Purpose

   Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   Non-targeting sgRNA human (DNMT3A S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-A3A

   Plasmid

   #193638

   Purpose

   Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3s-Sniper-Cas9

   Plasmid

   #113912

   Purpose

   Expresses Sniper-Cas9 with CMV promoter

   Depositor

   Jungjoon Lee

   Article

   Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

   Insert

   Sniper-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-3xFlag-dCas13-mTET2HxDCD

   Plasmid

   #228235

   Purpose

   For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

   Depositor

   Chuan He

   Article

   Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

   Inserts

   dCas13
   TET2 (Tet2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   CAG

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CARPID dCasRx-BASU

    Plasmid

    #153303

    Purpose

    express CARPID dCasRx-BASU fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    dCasRx-BASU
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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