We narrowed to 43,166 results for: cha

1. pcDNA 3.1 SIRT1-deltaExon2

   Plasmid

   #105671

   Purpose

   Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MCUabbb-Flag-PLYS1

   Plasmid

   #236787

   Purpose

   MCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.

   Depositor

   Dipayan Chaudhuri

   Article

   Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

   Insert

   MCUB, MCU mixed tetramer (MCU, MCUB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDual

   Plasmid

   #207883

   Purpose

   Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Inserts

   Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
   Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Leucine 212 changed to proline, leucine 434 in th…

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cav2.2 HA EIVA (E1658A) pCAGGS

   Plasmid

   #206082

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV/B-actin

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cav2.2 HA EIA (E314A) pCAGGS

   Plasmid

   #206081

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E314A

   Promoter

   CMV/B-actin

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Cav2.2 HA EIVK (E1658K) pcDNA3

   Plasmid

   #206066

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658K

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cav2.2 HA EIVK (E1658K) pCAGGS

   Plasmid

   #206096

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658K

   Promoter

   CMV/B-actin

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

   Plasmid

   #105681

   Purpose

   Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   tac_Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

   Plasmid

   #105680

   Purpose

   Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   tac_Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid

    #98598

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid

    #98599

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMiR-CIP2A-stop-mut

    Plasmid

    #53713

    Purpose

    Luciferase reporter assay for CIP2A with mutated firefly stop codon

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on the stop codon of firefly luciferase …

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Lenti-mCherry-WT-KRAS-IRES-Blast

    Plasmid

    #153335

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-WT-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-mCherry-G12V-KRAS-IRES-Blast

    Plasmid

    #153336

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-G12V-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-DIO-ASAP3Kv-WPRE

    Plasmid

    #132330

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3KV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLgw ELK4-V5-EcoDam

    Plasmid

    #98607

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSITEP-puro-shWRN1

    Plasmid

    #125783

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-puro-shRFP

    Plasmid

    #125778

    Purpose

    constitutive expression of a short-hairpin RNA targeting RFP

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSITEP-puro-shWRN1-C911

    Plasmid

    #125784

    Purpose

    a seed-matched control for pRSITEP-puro-shWRN1

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1-C911 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSITEP-puro-shWRN2

    Plasmid

    #125785

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN2 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge