We narrowed to 5,111 results for: codon optimized

1. pGEX6p1-GST-Rnf31

   Plasmid

   #63134

   Purpose

   Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

   Depositor

   Titia Sixma

   Article

   Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

   Insert

   Rnf31 (RNF31 Human, Synthetic)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synthetic based on human protein sequence

   Promoter

   tac

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Nsp15

   Plasmid

   #165120

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp15 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExodus CMV.Trex2

   Plasmid

   #40210

   Purpose

   Mammalian expression of codon-optimized Trex2.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Three prime repair exonuclease 2 (Trex2 Mouse)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-hPhyB-mCherry-HRasCT

   Plasmid

   #100281

   Purpose

   Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nsp14-EGFP

   Plasmid

   #165119

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p28-2iDP-puro

   Plasmid

   #88893

   Purpose

   Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dual-intron DMD platform
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   Truncated version of the dystrophin protein (tran…

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HF-BE3

    Plasmid

    #87439

    Purpose

    Expresses HF-BE3 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
    

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHS1-M7A,H102A

    Plasmid

    #159171

    Purpose

    Human codon-optimized cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    hHS1-M7A,H102A
    

    Expression

    Mammalian

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid

    #198551

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nsp10-mCherry

    Plasmid

    #165135

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp10 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2440

    Plasmid

    #72250

    Purpose

    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. YCplac33-NAT_MCART1

    Plasmid

    #140591

    Purpose

    Express MCART1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae

    Promoter

    NDT1 promoter and 3'UTR

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-hGTB

    Plasmid

    #26196

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Mutation

    F2A has 2A cleavage site deleted, producing htTA-…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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