We narrowed to 4,994 results for: mcs

1. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

   Plasmid

   #179905

   Purpose

   Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

   Insert

   SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

   Promoter

   CAG

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINTO-N3::hTET3 HxD

   Plasmid

   #136365

   Purpose

   Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   HxD (H1077Y, D1079A)

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNA5-FRT/TO-FLAG-TONSL

   Plasmid

   #221011

   Purpose

   mammalian expression vector of Flag tagged TONSL siRNA resistant

   Depositor

   Daniel Durocher

   Article

   O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

   Insert

   TONSL (TONSL Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

   Plasmid

   #188763

   Purpose

   Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   NCOA3 (NCOA3 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

   Plasmid

   #156415

   Purpose

   kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mTagBFP2

   Mutation

   mutant KRAS4A-G12D

   Promoter

   kdrl

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIS-MDH1-HIS

   Plasmid

   #184558

   Purpose

   Bacterial expression of human MDH1 with HIS Tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Malate dehydrogenase 1 (MDH1 Human)
   

   Tags

   6xHIS

   Expression

   Bacterial

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

   Plasmid

   #180468

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   KRAS4A (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   V5-mScarlet-I

   Promoter

   kdrl

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

   Plasmid

   #156414

   Purpose

   kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Mutation

   mutant KRAS4B-G12V

   Promoter

   kdrl

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE

   Plasmid

   #188760

   Purpose

   Lentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   FOXO3 FOXO-TAD (FOXO3 Human)
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE

    Plasmid

    #188761

    Purpose

    Lentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    SMARCA2 QLQ (SMARCA2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE

    Plasmid

    #188762

    Purpose

    Lentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    KIBRA WW (WWC1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT236 lenti pEF-rTetR(SE-G72P)-3XFLAG-CXXC1 PHD-T2A-mCherry-BSD-WPRE

    Plasmid

    #188764

    Purpose

    Lentiviral backbone containing CXXC1 PHD as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    CXXC1 PHD (CXXC1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV5 lenti pEF-rTetR(SE-G72P)-3XFLAG-APBB1(WW)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188751

    Purpose

    Lentiviral backbone containing APBB1 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    APBB1 WW (APBB1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMV6 lenti pEF-rTetR(SE-G72P)-3XFLAG-CRTC2(TORC-C)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188752

    Purpose

    Lentiviral backbone containing CRTC2 TORC_C domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    CRTC2 TORC_C (CRTC2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE

    Plasmid

    #188753

    Purpose

    Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    CACO1 Zn-C2H2_12 (CALCOCO1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. t1-405(151-154AAAA)

    Plasmid

    #166131

    Purpose

    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(151-154AAAA) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    aa1-405 only. Contains 4 amino acid substitutions…

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUb FLAG-tws

    Plasmid

    #195068

    Purpose

    Expresses FLAG-tagged Drosophila tws protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    twins (tws Fly)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Ubi-p63e

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-MP2

    Plasmid

    #36097

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGolden-AAV

    Plasmid

    #51424

    Purpose

    Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    lacZ
    

    Use

    AAV

    Promoter

    lacZ

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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