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p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Flag Depdc5 pLJM1

Plasmid

#46336

Depositor

David Sabatini

Publication

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMXs-puro GFP-UVRAG

Plasmid

#38267

Depositor

Noboru Mizushima

Publication

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV radiation resistance-associated gene protein (UVRAG Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
hPGK-rtTA3

Plasmid

#195883

Purpose

Lentiviral construct of hPGK driven rtTA3

Depositor

Elaine Fuchs

Publication

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Publication

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

No Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-Opt13

Plasmid

#61698

Purpose

Expresses PTDH mutant Opt13

Depositor

Huimin Zhao

Publication

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt13

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Publication

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

Plasmid

#47542

Purpose

engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

Depositor

Henry Lester

Publication

Frazier et al J Biol Chem. 2013 May 29.

Insert

Glutamate-gated chloride channels

Expression

Mammalian

Mutation

retention motif in ER (starting at R340) changed …

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pdCas9-GFP-VPR

Plasmid

#181907

Purpose

Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

Depositor

Karsten Rippe

Publication

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9-VPR

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-WT

Plasmid

#82030

Purpose

Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Publication

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutations making the cDNA resistant to a siRNA

Promoter

CMV-tet

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL3Basic-miR126-EGFL7-Promoter

Plasmid

#32244

Depositor

Charles Lowenstein

Publication

Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

Insert

miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Promoter region 5' UTR

Promoter

5' UTR

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

Plasmid

#90013

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-Dicer

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Publication

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-Smad7

Plasmid

#80893

Purpose

mammalian expression of Smad7

Depositor

Aristidis Moustakas

Insert

Smad7 (Smad7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

aa 2-426

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLV.SP146.gp91.eGFP.cHS4

Plasmid

#30476

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP/cHS4

Use

Lentiviral

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_c-SRC

Plasmid

#171850

Purpose

pEGFP-N1_c-SRC

Depositor

Michele Pagano

Publication

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

c-SRC (SRC Human)

Tags

GFP

Expression

Mammalian

Mutation

Added a linker sequence in 3' of c-SRC: GSGS

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Publication

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Gateway Cloning

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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