We narrowed to 9,512 results for: try
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pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNonePromoterNoneAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…ExpressionPlantAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNB1
Plasmid#192301PurposeGateway entry vector for an inducible 3xFLAG-KPNB1DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-hCMV1-hKLF2-IV
Plasmid#60433Purposetet-inducible human KLF4DepositorInsertKLF2 (KLF2 Human)
ExpressionMammalianAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266E
Plasmid#161521PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editingDepositorInsertzSpRYCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…Tags3X FLAG, NLSExpressionPlantAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT
Plasmid#154281PurposeEntry vector encoding non-tagged O-GlcNAc TransferaseDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2MutationWT OGTAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL
Plasmid#209047PurposeGateway-compatible entry vector, with insert of the full length TAF5 geneDepositorInsertTAF5 full length
UseGateway entry vectorExpressionMammalianAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA
Plasmid#104097PurposeExpresses TREAT-PK co-localization control in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2 stem loops in 3'UTR and 24xPP7 stem l…ExpressionMammalianAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A: D175A D28A D9A)
Plasmid#90090PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cellsDepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…Available SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
JE301: pMVP (L5-L4) Tir1-P2A-AID
Plasmid#121722PurposepMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.DepositorInsertosTir1-P2A-AID (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc (C-term on osTIR1)Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-hKLF4
Plasmid#60435PurposeOver-expression of human KLF4DepositorInsertKLF4 (KLF4 Human)
ExpressionMammalianAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-3XFLAG
Plasmid#105784PurposeExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-3XFLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265F2
Plasmid#164722PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)NG-UGI
UseCRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…ExpressionPlantAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC8 D101E
Plasmid#224350PurposeExpresses human KDAC8 (HDAC8) D101E in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionInsectMutationAspartate 101 mutated to glutamatePromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
