We narrowed to 21,801 results for: Sis

1. pAAV-FLEX-SaCas9-U6-sgGrin2b

   Plasmid

   #124851

   Purpose

   Mutagenesis of Grin2b

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2b (Grin2b Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSUMO1 GFP

   Plasmid

   #118360

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. aMHC-puro rex-neo

   Plasmid

   #21230

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNAD1

   Plasmid

   #154347

   Purpose

   pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

   Depositor

   John van der Oost

   Article

   Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

   Insert

   ShBle
   

   Use

   Microalgae

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Violaxanthin Chlorophyll a Binding Protein Precur…

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SBC015866

   Plasmid

   #226280

   Purpose

   Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

   Depositor

   Rainer Breitling

   Article

   Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

   Inserts

   4'-phosphopantetheinyl transferase Sfp
   Cinnamyl alcohol dehydrogenase
   Carboxylic acid reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. FKBP-beta1(617-949)-GFP

   Plasmid

   #100732

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP1B1 (AP1B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP/golgin-160/3DE

   Plasmid

   #180631

   Purpose

   Expression of caspase-resistant golgin-160

   Depositor

   Carolyn Machamer

   Article

   Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

   Insert

   golgin-160/3DE (GOLGA3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D59E, D139E, D311E

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

   Plasmid

   #124860

   Purpose

   Mutagenesis of Slc17a6

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-PGK-EGFPc3-Neo

    Plasmid

    #189741

    Purpose

    The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC32B-AtTAS1c-D2-B/c

    Plasmid

    #137884

    Purpose

    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Insert

    AtTAS1c-D2-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    2x35S

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. aMHC-Puro Rex-Blast

    Plasmid

    #21231

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST_6xUAS:Lamp1-mGFP

    Plasmid

    #192356

    Purpose

    Lamp1 tagged with mGFP, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Lamp1 (LAMP1 Human)
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Use

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPKm-248

    Plasmid

    #90504

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PDZb-beta2(616-951)-mCherry

    Plasmid

    #100736

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    PDZb and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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