We narrowed to 22,705 results for: his

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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

Plasmid

#217321

Purpose

Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

Depositor

Yifan Wang

Insert

NLRP1

Tags

FLAG and His-TEV

Expression

Insect

Mutation

S969A

Promoter

polyhedrin

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

Plasmid

#221896

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (450-517)-ULK1 (836-1050)-His

Plasmid

#222825

Purpose

Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His and MBP

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His and MBP

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#222822

Purpose

Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His-GST

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; 6xHis-GST-TEV, low expression levels

Plasmid

#204804

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z

Plasmid

#148159

Purpose

Insect Expression of Dm4E-T_1-511-L36AL69A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-L36AL69A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

Plasmid

#192835

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site and 6xhis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

Plasmid

#185701

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(FCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

Plasmid

#185705

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PDCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

Plasmid

#185703

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(SCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

Plasmid

#185704

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(PCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

Plasmid

#185702

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

anCas(BCA)

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

Plasmid

#162957

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

Plasmid

#162955

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

Plasmid

#162954

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

Plasmid

#162934

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

Plasmid

#162933

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-maltose binding protein fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

Plasmid

#166854

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

F641E, I645E

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

Plasmid

#166853

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

F641G, I645G

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

Plasmid

#153441

Purpose

E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc. XDocB binding mode B knock-out mutant

Tags

3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

Expression

Bacterial

Mutation

Arginine 140 and methionine 144 were mutated to a…

Promoter

T7 promoter

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

Plasmid

#153440

Purpose

E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc. XDocB binding mode A knock-out mutant

Tags

3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

Expression

Bacterial

Mutation

Arginine 191 and leucine 195 on XDocB were mutate…

Promoter

T7 promoter

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

Plasmid

#92227

Purpose

Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP Y35TAG D193TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Residues Y35 D193 were mutated into TAG (Amber) s…

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

Plasmid

#92319

Purpose

Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

Plasmid

#92318

Purpose

Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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