We narrowed to 24,603 results for: Nes

1. pTRANS_222

   Plasmid

   #91116

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-P12

   Plasmid

   #120023

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet
   

   Use

   Pcr template

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P06

   Plasmid

   #120017

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   sfGFP
   

   Use

   Pcr template

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRANS_223

   Plasmid

   #91117

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221-CAG.Pro

   Plasmid

   #79490

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CAG Promoter
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   CAG Promoter

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_D10A-1x7

   Plasmid

   #58777

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Carl-ReNL-KDEL_pcDNA3

   Plasmid

   #89535

   Purpose

   Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJG80

   Plasmid

   #89282

   Purpose

   Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-Z28

   Plasmid

   #120071

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xHA
   

   Use

   Pcr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBFP (GB0025)

    Plasmid

    #68193

    Purpose

    Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    BFP coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Carl-YeNL-KDEL_pcDNA3

    Plasmid

    #89516

    Purpose

    Yellow color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEP112E

    Plasmid

    #97398

    Purpose

    binary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Mitochondria target

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMaCTag-Z21

    Plasmid

    #120064

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Carl-CeNL-KDEL_pcDNA3

    Plasmid

    #89509

    Purpose

    Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-1-EXOB-11

    Plasmid

    #197688

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    EXOB enhancer-blocking insulator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN_TT 3xFLAG Luciferase

    Plasmid

    #98391

    Purpose

    Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    TRE tight and TRE tight promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCGB

    Plasmid

    #135283

    Purpose

    GBPart - CGB Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Click Beetle Green Luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TET-pLKO.1 PURO shLuc

    Plasmid

    #83092

    Purpose

    Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LAMP-OeNL_pcDNA3

    Plasmid

    #89525

    Purpose

    Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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