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pLEX_305-N-dTAG

Plasmid

#91797

Purpose

Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1c

Plasmid

#163682

Purpose

Far-red fluorescent calcium indicator FR-GECO1c

Depositor

Robert Campbell

Article

Dalangin et al bioRxiv 2020.11.12.380089

Insert

FR-GECO1c

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mitoDsRed

Plasmid

#44386

Purpose

3rd generation lentiviral vector

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoDsRed

Use

Lentiviral

Tags

61 aa targeting sequence of the P1 isoform F1F0-A…

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2631

Plasmid

#144140

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

INSM1 (INSM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_USP10

Plasmid

#127102

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Expression

Mammalian

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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